在被动表达的汗液中对细胞因子进行时间分析,以检测感染,使用 AWARE 设备
Preeti Singh1, Akash Kumar1, Kundan Kumar Mishra1
1Department of Bioengineering, University of Texas at Dallas, Richardson, Texas 75080, United States.
ACS sensors
|August 1, 2025
概括
这项研究开发了一种可穿戴传感器,使用阿普塔默来非侵入性检测炎症生物标志物瘤坏死因子-α (TNF-α) 和汗水中的互白素-6 (IL-6). 该AWARE-SENSOR提供精确的,实时监测免疫反应和生理压力.
科学领域:
- 生物医学工程 生物医学工程
- 生物传感器开发开发
- 可穿戴技术可穿戴技术
背景情况:
- 支持炎症的细胞因子,瘤坏死因子-α (TNF-α) 和介乐金-6 (IL-6) 是免疫反应,炎症和生理压力的关键生物标志物.
- 目前用于检测细胞因子的方法通常需要侵入性程序,或者不适合持续监测.
- 由于其稳定性,特异性和合成性质,aptamers在生物传感器应用中比抗体具有优势.
研究的目的:
- 开发和验证一个可穿戴传感器,AWARE-SENSOR,用于在人类汗水中非侵入性检测TNF-α和IL-6.
- 在实时生物传感平台中利用aptamer技术提高稳定性和特异性.
- 能够持续监测炎症生物标志物,以评估免疫状态和生理压力.
主要方法:
- 测试用于TNF-α和IL-6检测的可穿戴口味传感器 (AWARE-SENSOR) 的开发.
- 使用酸盐缓冲盐水 (PBS) 和人类汗水样本进行基准验证.
- 基于对目标细胞因子的aptamer结合的电化学检测,改变界面电特性.
主要成果:
- 传感器显示TNF-α的动态范围为0.1-200 pg/mL,IL-6的动态范围为1-256 pg/mL.
- 实现了高灵敏度,TNF-α的检测极限为2.77 pg/mL,IL-6的检测极限为3.22 pg/mL.
- 在大约3分钟内,AWARE-SENSOR准确地检测到人类汗液中的生物标志物,没有显著的交叉干扰.
结论:
- AWARE-SENSOR是一个基于aptamer的新型可穿戴设备,用于对汗水中的关键炎性细胞因子进行非侵入性实时监测.
- 在可穿戴生物传感器应用中,aptamer选择为抗体提供了稳定和特定的替代方案.
- 这项技术对持续的健康监测,早期疾病检测和个性化压力评估具有前景.
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