相关实验视频

Updated: Sep 13, 2025

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

Published on: January 23, 2018

DA-9701 (Motilitone®) 通过保护STZ诱导的β细胞死亡来改善葡萄糖平衡

Yurim Hong¹, Juhee Kim², Yeon-Gyeong Kim¹

¹Institute of Medical Research, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, the Republic of Korea.

Biochemical and biophysical research communications

|August 1, 2025

概括

DA-9701是一种植物药物,通过保护β细胞质量和功能,显示出对糖尿病治疗的前景. 它减少了β细胞死亡,改善了糖尿病模型中的葡萄糖平衡,而不会影响正常状况.

科学领域:

内分泌学在内分泌学.
药理学药理学是指药理学的学科.
细胞生物学细胞生物学

背景情况:

贝塔细胞死亡有助于胰岛素缺乏和高血糖在糖尿病 (DM).
预防β细胞亡是改善DM中的葡萄糖恒温的潜在策略.
DA-9701 (Motilitone®) 是一种已批准用于功能性消化不良的植物药物.

研究的目的:

研究DA-9701在维护β细胞质量的机制及其对糖尿病的治疗潜力.
评估DA-9701对糖尿病小鼠模型中的葡萄糖恒温和β细胞亡的影响.

主要方法:

DA-9701 已被施用于链毒素 (STZ) 诱导的糖尿病和肥胖小鼠.
评估了β细胞质量,分裂的caspase-3表达和葡萄糖平衡.
此外,还对用STZ.治疗的MIN6细胞进行了实验.

主要成果:

在STZ诱导的糖尿病小鼠中,DA-9701改善了葡萄糖平衡,并增加了β细胞质量.
DA-9701降低了切割的caspase-3表达,表明了抗亡作用.
在抗胰岛素的肥胖小鼠中,DA-9701增加了β细胞质量和胰岛素水平,但没有改善葡萄糖耐受性.
DA-9701的保护作用是特定于病理状况,而不是正常状态.

结论:

在STZ模型中,DA-9701主要通过减少β细胞亡来改善葡萄糖平衡.
由于其已确立的安全性,DA-9701是作为糖尿病治疗中的β细胞保护剂的有希望的候选人.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.DA-9701 是一个DA-9701 糖尿病是一种糖尿病.β细胞质量质量

更多相关视频

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

Published on: May 2, 2025

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

Published on: May 10, 2018

相关概念视频

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...

Hormones Regulating Blood Glucose

Hormones Regulating Blood Glucose

Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...

Glucose Homeostasis: Regulation of Blood Glucose

Glucose Homeostasis: Regulation of Blood Glucose

Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...

Oral Hypoglycemic Agents: Biguanides and Glitazones

Oral Hypoglycemic Agents: Biguanides and Glitazones

Biguanides, particularly metformin (Glucophage), are insulin sensitizers that enhance glucose uptake, thereby reducing insulin resistance. Unlike sulfonylureas, metformin doesn't prompt insulin secretion, which helps to curb hypoglycemia risk. Metformin is beneficial in treating conditions like polycystic ovary syndrome due to its insulin-resistance reduction capability. The drug's primary action involves curtailing hepatic gluconeogenesis, a significant contributor to high blood...

Hypoglycemia and Glucagon

Hypoglycemia and Glucagon

Without prolonged fasting, healthy individuals maintain blood glucose levels above 3.5 mM due to a well-adapted neuroendocrine counterregulatory system that effectively prevents acute hypoglycemia, a potentially life-threatening condition. The primary clinical scenarios for hypoglycemia encompass diabetes treatment, inappropriate production of endogenous insulin or insulin-like substances by tumors, and the use of glucose-lowering agents in non-diabetic individuals. Notably, hypoglycemia in the...

Pathophysiology of Diabetes

Pathophysiology of Diabetes

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...