由于脂酶活性较弱,Listeria monocytogenes的错误阴性诊断的风险很大
Lauren V Alteio1, Ariane Pietzka2, Laura van den Höövel3
1FFoQSI GmbH - Austrian Competence Centre for Feed and Food Quality, Safety and Innovation, Technopark 1D, Tulln 3430, Austria.
Diagnostic microbiology and infectious disease
|August 1, 2025
概括
一种非典型的Listeria monocytogenes菌株具有减少的脂酶活性,可能会在食品安全诊断中引起虚假阴性. 这项研究发现了prfA基因突变,突出了改进李斯特菌检测方法的需要.
科学领域:
- 食品微生物学 食品微生物学
- 细菌遗传学 细菌遗传学
- 诊断方法 诊断方法
背景情况:
- 李斯特菌 (Listeria monocytogenes) 是一种重要的食物传播病原体.
- 非典型菌株可以逃避标准诊断测试.
- 在一些非典型菌株中观察到减少的脂酶活性.
研究的目的:
- 调查来自食品加工环境的非典型Listeria monocytogenes菌株.
- 确定脂酶活性降低的原因及其与殖民地出现的联系.
- 评估对当前诊断方法和食品安全的影响.
主要方法:
- 聚合酶连锁反应 (PCR) 用于物种确认.
- 矩阵辅助激光消耗/电离-飞行时间 (MALDI-TOF) 质谱学.
- 酶活性测定和比较基因组学.
- 对prfA基因和脂酶C基因的分析.
主要成果:
- 使用PCR和MALDI-TOF确认了非典型的李斯特菌单细胞原体.
- 减少的脂酶活性与染色体上的白色外观相关联.
- 在脂酶C基因中没有发现突变.
- 在prfA基因中插入了七个基对,导致了基因切断.
结论:
- 在Listeria monocytogenes中发现的prfA基因突变可能解释了病毒毒性降低和酶活性改变.
- 带有这种突变的非典型菌株在常规诊断试验中存在假阴性结果的风险.
- 改进的检测策略对于确保食品安全和公共卫生至关重要.
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