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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
252
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

127
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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相关实验视频

Updated: Sep 13, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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cliCRISPR:用于多重检测的crRNA有限的CRISPR/Cas12a系统.

Bo Yan1, Cong Wei2, Xueying Lei2

  • 1Department of stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

Biosensors & bioelectronics
|August 1, 2025
PubMed
概括

我们开发了cliCRISPR,这是一种使用有限crRNA进行多重核酸检测的新型CRISPR/Cas12a方法. 这种策略将光强度与crRNA度相关联,使精确的基因型和生物传感器开发成为可能.

关键词:
这就是CRISPR/Cas12a.核酸分析 核酸分析单个核酸的多态性.维生素D 维生素D是一种维生素.

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 遗传学 遗传学 是一个

背景情况:

  • 克里斯普尔/Cas12a被广泛用于核酸检测,通常需要多余的crRNA.
  • 克里斯普尔/Cas12a的非特异性跨裂变活性阻碍了单测定中的多重检测.
  • 现有的方法在实现敏感和特定的多重检测方面面临挑战.

研究的目的:

  • 通过CRISPR/Cas12a系统引入一个限于crRNA的策略,即cliCRISPR,用于使用CRISPR/Cas12a系统进行多重检测.
  • 为了证明光强度可以与多重量化的crRNA度相关.
  • 为了验证cliCRISPR方法用于精确的基因变异的基因定型.

主要方法:

  • 开发了一个限于crRNA的CRISPR/Cas12a策略 (cliCRISPR).
  • 与特定crRNA度相关的光强度,用于不同的信号水平.
  • 应用逻辑门策略进行基因定型rs4646536,这是一种与维生素D缺乏相关的变异.
  • 使用临床样本验证了该方法,并将结果与TaqMan qPCR进行了比较.

主要成果:

  • 使用受控crRNA度 (10nMcrRNA1,3nMcrRNA2) 达到可辨别的光强度.
  • 成功的基因型 rs4646536 (野生类型,突变,异构卵性) 的高精度.
  • 证明与TaqMan qPCR结果的一致性以及对孟德尔遗传模式的遵守.
  • 验证了cliCRISPR在家族样本中的多重基因定型的可行性.

结论:

  • cliCRISPR为多重核酸检测提供了一种新的crRNA有限的方法.
  • 该方法通过将光与crRNA度相关联,使得精确的基因型定型成为可能.
  • cliCRISPR显示出开发基于CRISPR/Cas12a技术的先进多重生物传感器的巨大潜力.