在Listeria monocytogenes中银纳米颗粒的耐药性:形态学,毒性和细胞反应
Juan Du1, Ziyue Yu1, Jing Jin1
1College of Food and Bioengineering, Zhengzhou University of Light Industry, Key Laboratory of Cold Chain Food Processing and Safety Control (Zhengzhou University of Light Industry), Ministry of Education, Zhengzhou 450001, China.
International journal of food microbiology
|August 1, 2025
概括
细菌正在对银纳米粒子 (AgNPs) 产生耐药性,这是一种关键的抗菌剂. 这项研究表明,Listeria monocytogenes通过细胞形状,毒性和运动性的独特变化,与以前已知的机制不同,发展出AgNPs耐药性.
科学领域:
- 微生物学 微生物学
- 纳米技术纳米技术
- 生物化学 生物化学
背景情况:
- 细菌抗生素耐药性是全球主要的健康问题.
- 银纳米粒子 (AgNPs) 由于其抗菌特性而广泛使用.
- 新兴的细菌对AgNPs的耐药性需要了解新的耐药性机制.
研究的目的:
- 为了研究Listeria monocytogenes中AgNPs耐药性的机制.
- 在抗AgNPs的细菌中的表型和遗传变化的特征.
- 阐明AgNPs耐药性如何影响细菌的毒性和运动性.
主要方法:
- 球形酸盐封顶的AgNP的合成 (大约. 12 nm) 的距离.
- 在Listeria monocytogenes中通过重复暴露诱导AgNPs耐药性.
- 对细胞形态,细胞壁厚度,基因表达 (差异和qRT-PCR) 和滑动动性的分析.
- 血液溶解试验验证基因表达变化.
主要成果:
- 李斯特菌对AgNPs (L.M_AgNPs) 产生了耐药性.
- 耐药菌株表现出明显的缩细胞极,细胞壁厚度增加了17.4%.
- 病毒性基因表达显著下调,一些基因表达最小.
- 在抗性菌株中,滑翔运动率显著增加.
结论:
- 在L. monocytogenes中,AgNPs的耐药性是由细胞形态变化 (渐变的极点),毒性降低和增强的滑翔机动性介导的.
- 这些机制不同于之前报告的AgNPs耐药性途径.
- 这些发现有助于更好地理解细菌应激反应,病毒性,并促进更安全的AgNP利用.
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