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对OsNCED3转基因大米的转录组分析揭示了对性压力的反应机制

Yang Xu¹, Zhonghui Feng², Guanru Lu³

¹State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China; Jilin Da'an Agro-Ecosystem National Observation Research Station, Da'an, 131317, China.

Plant physiology and biochemistry : PPB

|August 1, 2025

概括

在大米中过度表达OsNCED3基因通过提高酸 (ABA) 水平和抗氧化活性来提高性压力耐受性. 这种基因改造改善了根的生长,并在盐性土壤中显著增加了谷物产量.

科学领域:

植物生物学植物生物学
农业科学农业科学
环境压力生理学生理学

背景情况:

土壤化对全球作物生产构成重大威胁,特别是大米 (Oryza sativa).
了解性应激耐受性的分子机制对于提高受影响地区的产量至关重要.

研究的目的:

研究酸 (ABA) 合成基因9-cis-epoxycarotenoid dioxygenase 3 (OsNCED3) 在大米中赋予性应激耐受性的作用.
评估OsNCED3过度表达对生长,生理反应和性条件下的谷物产量的影响.

主要方法:

克隆和过度表达的OsNCED3基因在性耐药大米品种Dongdao-4,创建转基因线.
在性压力下评估根类表型,ABA含量,抗氧化酶活性 (SOD,CAT,POD,APX) 和活性氧物种 (ROS) 水平.
进行转录组分析以识别差异表达基因 (DEG) 并执行基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
进行农学调查以评估谷物产量.

主要成果:

与野生型 (WT) 植物相比,过度表达OsNCED3的转基因大米系表现出增强的根部发育和显著增加对性压力的耐受性.
OsNCED3的过度表达导致了更高的ABA含量和抗氧化酶的活动增加,同时降低了ROS和甲 (MDA) 的水平.
转录组分析揭示了参与植物激素信号转导和MAPK信号通路的基因的显著上调和下调.
农学评估表明,OsNCED3过度表达线的谷物产量大幅增加.

结论:

过度表达OsNCED3通过调节ABA生物合成,抗氧化剂防御系统和应激响应信号通路来增强大米对性压力的耐受性.
OsNCED3基因作为一种有价值的米基因改进目标,以提高盐环境中的生产力.

关键词:

性压力是一种性压力.在 OsNCED3 中.大米大米大米大米大米文字转录学 (Transcriptomics) 是一个学科.转基因的线条转基因的线条.

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