实时成像内源转录因子动态揭示了表皮质和原始内皮的命运分离机制
Rebecca P Kim-Yip1, David Denberg2, Denis F Faerberg3
1Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Current biology : CB
|August 1, 2025
概括
早期小鼠胚胎发育涉及细胞命运的决定. 一个初级表皮质细胞 (EPI) 谱系形成,通过FGF信号影响原始内皮体 (PE) 差异化,NANOG水平影响轨迹切换.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
背景情况:
- 哺乳动物早期发育中的细胞命运决定对于建立不同的细胞系至关重要.
- 在老鼠胚胎模型中,表皮质 (EPI) 和原始内皮 (PE) 的分离是预先确定的与出现的模式.
- 了解管理EPI/PE规范的分子机制是解读发育过程的关键.
研究的目的:
- 研究EPI和PE细胞命运确定过程中转录因子的动态.
- 阐明细胞命运模式中的血统历史和随机事件之间的相互作用.
- 模拟EPI/PE分离在植入前小鼠胚胎中的启动和传播.
主要方法:
- 内源标记转录因子的定量实时成像.
- 在命运决策过程中单细胞分辨率追踪细胞动态.
- 对SOX2,GATA6和NANOG表达动态的分析.
主要成果:
- 确定了一个初始对称性破坏事件,形成了一个与SOX2动态相关的初级EPI血统.
- 从初级EPI血统中展示的FGF信号诱导了在注定PE分化细胞中诱导GATA6.
- 揭示了NANOG表达水平可以使细胞轨迹偏向二次EPI或持续PE命运.
结论:
- EPI/PE分离是由血统历史偏差事件和随机分子特征的组合引起的.
- 早期发育模式涉及初始对称性破坏和随后的信号通路之间的相互作用.
- 这项研究通过整合EPI/PE命运决定中的血统历史和随机性来统一以前的模型.
关键词:
GATA6 GATA6 是一个数字.在ICM中使用ICM.这是一个NANOGNANOG.这就是SOX2的含量.胚胎细胞囊中的胚胎囊.经皮质细胞是什么?现场成像 现场成像 现场成像这里是鼠标鼠标鼠标鼠标鼠标鼠标.在植入前的胚胎胚胎.原始的内皮原始的内皮.更多相关视频
10:36In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
Published on: March 22, 2017
7.7K
13:03Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
Published on: June 3, 2016
8.2K
相关概念视频
01:56Gastrulation
58.6K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
58.6K
01:51Determination
19.1K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
19.1K