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化应激会影响Saccharomyces cerevisiae中的线粒体呼吸链复合II和复合IV组件:复合II的S-化
Sanchita Biswas1, Ayantika Sengupta1, Shubhojit Das2
1Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
Biochimica et biophysica acta. General subjects
|August 1, 2025
概括
氧化 (NO) 和反应性物种 (RNS) 引起化应激,抑制线粒体呼吸. 这项研究揭示了S-化无活化酵母复合体II并损害复合体III/IV的组合,影响细胞能量生产.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 线粒体生物学 线粒体生物学
背景情况:
- 较高的氧化 (NO) 和反应性物种 (RNS) 诱导细胞化应激.
- 已知酸性压力会抑制线粒体呼吸,但机制尚不清楚.
- 线粒体复合体II,III,IV和V在酵母和人类之间保存,使酵母成为一个相关的模型.
研究的目的:
- 在Saccharomyces cerevisiae中,研究在化应激下线粒体呼吸功能障碍的机制.
- 在体内确定NO和RNS作用的特定线粒体点.
- 了解酸性压力如何影响线粒体呼吸链复合体的组装和功能.
主要方法:
- 使用野生型和突变的Saccharomyces cerevisiae菌株进行微生物生长分析.
- 用酸 (SNP) 和S-Nitrosoglutathione (GSNO) 治疗以诱导化应激.
- 测量氧气消耗率,酶活性,蛋白质S-化,氨酸化,基因表达和超复杂组装通过蓝色本地PAGE和西方涂抹.
主要成果:
- 化应激显著影响了酵母细胞的生长和线粒体的氧气消耗.
- 发现复合II (苏酸脱酶) 是S-基化和无活化.
- 复杂III和IV活动被不可逆转地抑制,Sdh2和Cox2子单元的表达减少.
- 化应激导致本地复合II和含有复合III和IV的超级复合体的组装减少.
结论:
- 这项研究提供了第一个体内证据,证明了在酵母体中化应激下线粒体复合体的修饰.
- 复合II的S-化和呼吸链超复合体的受损组装是化应激诱导的线粒体功能障碍的关键机制.
- 在Saccharomyces cerevisiae中的发现提供了对与化压力相关的人类线粒体疾病的见解.
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