用于测量细胞和组织机械异性异性度的 toroidal 缩影
Juanyong Li1, Chaokai Zhang1, Habibeh Ashouri Choshali2
1Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA, USA.
Acta biomaterialia
|August 1, 2025
概括
这项研究引入了一种新的,低成本的 toroidal 缩影方法来测量生物材料的异性质刚度,从组织到细胞. 这种可访问的技术克服了传统方法的局限性,使得更广泛的生物力学研究成为可能.
科学领域:
- 生物力学 生物力学
- 细胞机械生物学 细胞机械生物学
- 生物材料科学 生物材料科学
背景情况:
- 入试验测量组织和细胞的刚性,但通常假定具有同位素材料的特性.
- 使用球形或金字塔形探针和赫兹分析的标准方法未能捕捉纤维组织和极化细胞的方向依赖性特性.
- 在没有先进的成像或专用设备的情况下,测量跨尺度的异性质机械性质一直是具有挑战性的.
研究的目的:
- 开发一种通用,可访问的缩方法,用于估计生物材料的异型弹性模块.
- 为了创建适合各种尺度,从毫米到微米的 torous 形的内置探头.
- 建立一个结合有限元建模和深度学习用于模块计算的计算框架.
主要方法:
- 制造具有不同尺寸比的 torous 形内探头.
- 不同类型的生物样本 (肌肉组织,细胞单层,单细胞) 沿着和垂直于纤维方向的入.
- 开发一个线性不可压缩的横向同位素材料模型和有限元素模拟.
- 在模拟数据上训练一个深度学习模型来计算异型模块 (E1和E2).
主要成果:
- 开发的状缩法成功估计了肌肉组织,细胞单层和单细胞的异性质模块.
- 测量的异构度 (E1:E2) 与这些生物系统之前公布的值保持一致.
- 该方法与现有技术具有可比性,同时更容易获得和更具成本效益.
结论:
- 状缩方法提供了一种通用,可访问和低成本的方法,用于跨度尺度测量生物材料中的异型刚性.
- 这种技术克服了传统缩方法中同otropic假设的局限性.
- 这项研究为推进组织工程,生物力学和机械生物学研究提供了宝贵的工具,因为它可以研究异型生物系统.
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