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Updated: Sep 13, 2025

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化EM捕捉了由LIS1激活dynein的早期中间步骤

Kendrick H V Nguyen1, Eva P Karasmanis1, Agnieszka A Kendrick2

  • 1Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, USA.

Nature communications
|August 1, 2025
PubMed
概括

由LIS1激活细胞质二烯-1 (二烯) 涉及到一个新的"Pre-Chi"中间状态. 这一发现揭示了LIS1如何调节细胞运输的dynein运动功能和组合.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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