在前列腺瘤模型中使用CEST MRI对谷氨胺的吸收和代谢进行成像
Yuki Hodo1, Caitlin M Tressler2, Behnaz Ghaemi2,3
1Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Npj imaging
|August 1, 2025
概括
化学交换和转移 (CEST) MRI 可以在临床前前列腺癌模型中可视化谷氨酸的摄取和代谢. 这种技术在区分具有不同谷氨酸活性的瘤方面表现有前途,有助于临床应用.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 在瘤学瘤学.
- 代谢成像 - 代谢成像
背景情况:
- 谷氨胺代谢在癌症中至关重要,但目前的成像剂有局限性.
- 化学交换和转移 (CEST) MRI提供了一种潜在的方法来克服这些局限性.
研究的目的:
- 调查使用CESTMRI用于在临床前前列腺癌模型中图像谷氨胺吸收和代谢的可行性.
- 评估CESTMRI在基于谷氨胺代谢活性区分瘤方面的潜力.
主要方法:
- 利用11.7T的CESTMRI检测幽灵和前临床前列腺癌模型 (LNCaP和DU-145细胞) 中的谷氨酸及其代谢物.
- 在3D细胞培养和体内瘤中服用谷氨酸和测量CESTMRI增强.
- 采用质谱成像来确认谷氨胺的摄取和代谢.
主要成果:
- CEST MRI检测到谷氨酸及其下游产物,在较低的pH值下对比度增加.
- 与DU-145细胞相比,LNCaP细胞表现出关键代谢蛋白质的更高表达,在输入谷氨酸后显示出持续更高的CEST MRI增强.
- 质谱成像证实了LNCaP瘤中较高的谷氨胺吸收和代谢.
结论:
- 在临床前模型中,胺的CEST MRI可以成功地绘制瘤吸收和代谢的图像.
- 这项技术证明了区分前列腺瘤模型与不同的谷氨胺代谢配置文件的能力.
- 谷氨胺的CESTMRI具有在癌症诊断和监测中临床翻译的潜力.
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