相关实验视频
Updated: Sep 13, 2025

07:44
Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
Published on: October 6, 2017
17.4K
规范的Wnt信号调节神经发生过程中Mbd3蛋白的稳定性
Nhu Thi Quynh Mai1, Soyoung Jeon2,3, Byoung-San Moon4
1Department of Medical Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
Experimental & molecular medicine
|August 1, 2025
概括
规范的Wnt信号稳定了Mbd3,这是神经原生细胞 (NPC) 恒温的关键调节器. 这种Wnt-Mbd3通路控制神经发生,为神经干细胞重编程提供了潜力.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 神经原生细胞 (NPC) 稳定性需要平衡自我更新和差异化才能进行正确的大脑发育.
- 规范的Wnt信号传递和Mbd3-NuRD复合体是神经发生的关键调节者.
- Mbd3-NuRD复合物对神经发生有抑制作用.
研究的目的:
- 调查Mbd3作为NPC中正规Wnt信号的下游目标.
- 阐明Wnt-Mbd3轴在调节神经发生和NPC干性的作用.
主要方法:
- 使用Wnt3a (激活器) 和DKK1 (抑制剂) 调节Wnt信号.
- 对β-catenin和Mbd3表达模式的分析.
- 过度表达和耗尽GSK3β以研究Mbd3无处不在.
- 评估Mbd3在通过NuRD复合组装抑制神经发生相关基因中的作用.
主要成果:
- Wnt信号调制与β-catenin和Mbd3表达的变化并行.
- GSK3β水平影响了Mbd3的无处不在,这表明Wnt信号稳定了Mbd3.
- 在Wnt-β-catenin的下游,Mbd3通过招募NuRD复合体来抑制神经发生基因,促进NPC干性.
结论:
- 一个新的Wnt-Mbd3信号轴调节了NPC中神经元细胞酸盐的确定.
- 这一途径通过抑制神经生成来增强NPC干性.
- Wnt-Mbd3通路代表了神经干细胞重编程和神经元分化的一个潜在的治疗点.
相关概念视频
Canonical Wnt Signaling Pathway
8.9K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.9K
Non-Canonical Wnt Signaling Pathways
7.4K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.4K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Notch Signaling Pathway
4.4K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.4K
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Hedgehog Signaling Pathway
7.5K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.5K

