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用Saccharomyces cerevisiae的代谢工程来生产新山丁

Natalia Arenas¹, Vicente F Cataldo², Eduardo Agosin^3,4

¹Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, 7820436, Santiago, Chile.

Microbial cell factories

|August 2, 2025

概括

这项研究报告了在酵母中首次成功的异质生产新山丁,通过代谢和酶工程实现了创纪录的产量. 这些进展为可扩展生产这种有价值的胡卜素铺平了道路.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

桑托菲尔,氧化胡卜素,具有抗氧化特性和健康益处,其中新桑显示治疗潜力.
尼奥桑丁是富可桑丁和β-达马森等有价值化合物的前体,用于化品和制药.
目前新山丁的生产方法有限,需要新的方法.

研究的目的:

在Saccharomyces cerevisiae中实现第一个异构的新山丁的生产.
通过代谢和酶工程策略优化新山丁产量.
建立一个可扩展的平台,用于neoxanthin生物合成.

主要方法:

通过表达来自β-胡卜素和紫素生物合成途径的基因来改造Saccharomyces cerevisiae.
引入了来自Phaeodactylum tricornutum的VDL1基因,将紫素转化为新素.
实施了脉冲食银河糖策略,并结合了跨膜来增强新山丁的积累.

主要成果:

实现了0.18 mg/g_DCW的初始新山丁产量.
使用脉冲养银河糖策略,提高了2.5倍的产量.
通过加入跨膜来进一步提高产量3.8倍,最终产量达到0.7 mg/g_DCW.

结论:

这项研究报告了迄今为止由工程微生物产生的新山丁的最高产量.
采用的策略显示出扩大新山丁生产的巨大潜力.
这项工作为生物技术生产neoxanthin提供了一个强大的平台.

关键词:

这种植物是Saccharomyces cerevisiae.胡卜素是一种胡卜素.代谢工程是代谢工程.新氧丁是一种新氧丁.桑托菲尔 (Xanthophyll) 是一种植物.

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