低氧气的可用性增加了Ustilago可能disdis的伊塔科纳酸生产
Marianne Volkmar1, Wolfgang Laudensack1, Felix Bartzack1
1Institute of Bioprocess Engineering, University of Kaiserslautern‑Landau, Kaiserslautern, Germany.
Biotechnology and bioengineering
|August 2, 2025
概括
减少 itaconic 酸生产中的能源消耗是关键. 低氧水平令人惊地提高了Ustilago maydis的伊塔康酸产量,提供了一个高效的工业生物技术战略.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 聚合物科学 聚合物科学
背景情况:
- 伊塔康酸是高性能聚合物的重要单体.
- 目前的工业生产使用的是Aspergillus terreus;Ustilago maydis是一个较新的替代品.
- 在U. maydis的有氧栽培中,由于高的气化和,它需要大量的能量.
研究的目的:
- 调查降低 itaconic 酸生产过程中能源消耗的策略.
- 探索氧气供应对U. maydis. 产生的伊塔康酸产生的影响.
- 确定过程控制策略,以提高工业规模的生物技术生产.
主要方法:
- 在不同氧气条件下种植Ustilago maydis.
- 从0.4L扩展到30L的生物反应器的实验.
- 在不同氧气可用性下分析伊塔克酸标位和产量.
主要成果:
- 低氧气可用性显著增加了伊塔克酸标位和产量.
- 这一发现与典型的有机酸发酵和先前关于U. maydis氧气敏感性的报道形成鲜明对比.
- 扩大规模的研究证实了限制氧气对伊塔科尼酸生产的积极影响.
结论:
- 限制氧气的可用性可以作为一个过程控制策略,以提高U. maydis.中 itaconic 酸生产的杆.
- 这种方法为提高工业规模生物技术过程的效率提供了一种新方法.
- 在低氧条件下,U. maydis的代谢途径似乎会转移,有利于伊塔康酸的合成.
相关概念视频
Oxygen Requirements and Growth Patterns
241
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
241
The Electron Transport Chain
17.3K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
17.3K
Inorganic Nitrogen Assimilation
106
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
106
Fates of Pyruvate
9.0K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
9.0K
Fermentation
117.7K
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
117.7K
Feedback Inhibition
54.6K
Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
54.6K


