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优化来自Vibrio proteolyticus的细胞外产品,以便在水产养殖中作为后生物制剂使用

Jorge García-Márquez¹, Marta Domínguez-Maqueda¹, Olivia Pérez-Gómez¹

¹Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Instituto Andaluz de Biotecnología y Desarrollo Azul (IBYDA), Campus Universitario de Teatinos S/N, 29071, Málaga, Spain.

Marine biotechnology (New York, N.Y.)

|August 2, 2025

概括

来自益生菌细菌Vibrio proteolyticus的细胞外产物显示出作为水产料添加剂的前景. 这些化合物增强鱼类的消化,表现出抗菌和抗生物膜的活动,并减少病原体的毒性,支持鱼类的健康和抗病能力.

科学领域:

水产养殖是水产养殖的一种方式.
微生物学微生物学
鱼类健康鱼类健康

背景情况:

维布里奥蛋白质是一种潜在的鱼类益生菌.
细胞外产品 (ECP) 影响鱼类的健康和抗病能力.
优化ECP的培养条件对于水产养殖应用至关重要.

研究的目的:

研究培养条件对V. proteolyticus ECPs的影响.
评估ECP的酶,抗菌,细胞毒性和生物膜调节活动.
评估ECP对鱼的肠道微生物群和病原体毒性的影响.

主要方法:

酶分析 (氨酶,氨酶,原酶).
针对鱼类病原体的抗菌分析.
用鱼类细胞系进行细胞毒性测定.
生物膜抑制测定.生物膜抑制测定.
短链脂肪酸 (SCFA) 的分析.
基因表达分析 (aip56).

主要成果:

环保蛋白表现出多样化的水解活性,抑制了主要的鱼类病原体 (P. damselae),并调节了生物膜的形成.
细胞毒性有所变化,一些细胞系表现出增加的活力.
SCFA 档案显示了潜在的抗微生物和肠道健康益处.
经过ECP显著减少了P. damselae亚种的数量. 通过降低 aip56.5 的活性,降低了 piscicida 的毒性.

结论:

V. proteolyticus ECP是潜在的水产料添加剂,可以改善鱼类的营养,健康和抗病能力.
进一步的研究应该集中在分离生物活性化合物和理解它们的作用机制上.
优化ECP应用可以使水产养殖和其他生物技术领域受益.

关键词:

维布里奥蛋白质溶解菌维布里奥蛋白质溶解菌细胞外产品细胞外产品微生物培养媒介.后生物的后生物是什么?益生菌是一种益生菌.

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