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Updated: Sep 13, 2025

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单细胞多组学揭示了细胞周期期间mRNA代谢和染色质可访问性的振荡动态

Maulik K Nariya¹, David Santiago-Algarra¹, Olivier Tassy¹

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Illkirch, France; Centre National de la Recherche Scientifique (CNRS), UMR 7104, Illkirch, France; Institut National de Santé et de Recherche Médicale (ISERM), UMR-S 1258, Illkirch, France; Université de Strasbourg, IGBMC UMR 7104-UMR-S 1258, Illkirch, France.

|August 2, 2025

概括

这项研究绘制了细胞周期基因表达动态图,揭示了mRNA转录和处理如何振荡. 转录后调节显著影响细胞分裂期间的mRNA水平.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
细胞生物学细胞生物学

背景情况:

细胞周期需要精确的时间基因表达.
整个细胞周期的全基因组mRNA代谢动态在很大程度上没有特征.

研究的目的:

在整个细胞周期中量化全基因组mRNA代谢率 (转录,拼接,输出,降解).
确定控制细胞增殖期间基因表达动态的调控机制.

主要方法:

单细胞多基因组测序
生物物理建模生物物理建模
深度学习是一种深度学习.
对染色质可访问性和转录因子结合足迹的分析.

主要成果:

在特定细胞周期阶段观察到转录和后转录过程中的明显振荡波.
转录后调节被确定为塑造mRNA积累的关键因素.
染色质可访问性和转录因子结合的动态变化与mRNA动态有关.

结论:

在细胞周期期间生成了RNA代谢和染色质可访问性动态的高分辨率地图.
揭示了对增殖细胞中基因表达的时间控制的新见解.

关键词:

(转录后) 转录后动力学科普:分子生物学分子生物学细胞循环中的细胞循环.染色体可访问性的动态.基因表达的动态基因表达的动态 mESCs是什么意思 mRNA新陈代谢 mRNA新陈代谢单细胞多组体的多组体.轨迹推断的推断是指轨迹的推断.

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