相关实验视频

Updated: Sep 13, 2025

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

该miR-122/P53/TIGAR信号轴调节由聚乙烯纳米塑料颗粒诱导的葡萄糖代谢重编程

Ting Chen¹, Changyong Gong¹, Fan Yu¹

¹Institute of Life Sciences & Biomedicine Collaborative Innovation Center of Zhejiang province, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.

Journal of hazardous materials

|August 2, 2025

概括

聚乙烯纳米塑料 (PS-NP) 通过改变miR-122/P53/TIGAR通路来破坏葡萄糖代谢. 这项研究阐明了纳米塑料诱导的代谢障碍背后的分子机制.

科学领域:

环境毒理学环境毒理学
分子生物学分子生物学
代谢学代谢学代谢学

背景情况:

聚乙烯纳米塑料 (PS-NP) 是普遍存在的环境污染物.
已知PS-NPs会引起毒性,包括代谢中断.
导致PS-NP毒性的精确分子机制尚未完全理解.

研究的目的:

阐明PS-NPs影响葡萄糖代谢的分子机制.
研究miR-122/P53/TIGAR信号轴在PS-NP诱导的代谢变化中的作用.
在体内和体外探索PS-NP对葡萄糖代谢途径的影响.

主要方法:

使用斑马鱼幼虫和Hep G2细胞系进行体内和体外研究.
采用了转录学,代谢学和分子生物学技术 (例如,RNA干扰,模仿/抑制剂测试).
分析了氧化应激,肝损伤,基因表达 (miR-122,P53,TIGAR) 和代谢途径转移 (EMP,PPP).

主要成果:

暴露PS-NP诱导了斑马鱼的氧化应激和肝损伤.
PS-NP显著降低了miR-122的调节,导致P53和TIGAR的调节.
通过miR-122/P53/TIGAR轴,PS-NP破坏了葡萄糖代谢,将其从糖溶性 (EMP) 转移到酸途径 (PPP).

结论:

这项研究揭示了通过miR-122/P53/TIGAR信号轴的PS-NP诱导的葡萄糖代谢重编程的新型分子机制.
这些发现为纳米塑料如何影响细胞代谢提供了关键的见解.
了解这种途径可以增强对纳米粒子暴露反应中的microRNA功能的知识.

关键词:

葡萄糖代谢重编程葡萄糖代谢重编程据了解,米R-1222号飞机是一架空中飞机.在P53中,P53是P53 这是PS-NPs.葡萄糖溶解途径的使用.酸酸盐通路中的酸.

更多相关视频

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

相关概念视频

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...