一种可靠的UHPLC-MS/MS方法,用于在人类尿液中确定遗留和新出现的双相似物
Xiao Lin1, Dejun Bao1, Qi Sun1
1China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, PR China.
Journal of chromatography. A
|August 2, 2025
概括
一种新方法准确地测量了尿液中的BPA和其他11种双类型. 在年轻人中发现了高暴露率,突出了这些广泛使用的化学物质对公共卫生的担忧.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 双化合物 (BPs) 在消费品中广泛使用.
- 暴露于BP是一种日益严重的公共卫生问题.
- 关于人类群体中新出现的双相似物存在有限的数据.
研究的目的:
- 开发和验证一种有效的方法来量化BPA,BPF,BPS,以及人类尿液中的11种新兴双相似物.
- 评估这些双相似物在北京年轻人群中的患病率和水平.
主要方法:
- 固相提取 (SPE) 结合超高性能液体染色学-并联质谱学 (UHPLC-MS/MS).
- 用β-葡萄糖酶进行水解,使用HLB 96井板进行丰富,并通过多重反应监测 (MRM) 模式进行分析.
- 验证包括线性,方法检测极限 (MDL),方法量化极限 (MQL) 和恢复研究.
主要成果:
- 该方法显示出良好的线性 (r > 0.999),低的MDL (0.01-0.07 ng/mL) 和可接受的恢复率 (72-127%).
- 在187个尿样中检测到BPA (98.9%) 和BPS (80.2%) 的高患病率.
- 新兴的替代DBSP在32.6%的样本中被检测到,这表明暴露很大.
结论:
- 开发的UHPLC-MS/MS方法是有效和可靠的,用于分析人类尿液中的双相似物.
- 在年轻人群中,传统和新兴双相似物广泛暴露.
- 调查结果强调需要继续监测和评估双暴露和潜在的健康风险.
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