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蛋白质组学分析揭示了新鲜果汁和加工果汁的差异:一个不是来自缩汁的情况
Lu Mi1, Kewen Wang1, Shini Yang1
1College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Institute of Quality Standard & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing 100081, China.
蛋白质组学揭示了新鲜和加工汁中的不同蛋白质配置. 处理,特别是巴氏杀菌,破坏细胞结构,改变蛋白质的丰富性,导致潜在的降解标志物.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 汁加工方法,如巴氏杀菌和高压加工,可以改变其生物化学成分.
- 了解蛋白质变化对于加工食品的质量控制和真实性评估至关重要.
研究的目的:
- 用比较蛋白质组学来区分新鲜和加工的汁.
- 为了识别差异丰富的蛋白质 (DAP) 和潜在的标记,表明加工.
主要方法:
- 对新鲜,消毒和高压加工的汁进行比较蛋白质组学分析.
- 双重质量标签 (TMT) 标签与纳米-UHPLC-Orbitrap Fusion MS.相结合.
- 基因本体学丰富分析和分子动力学模拟.
主要成果:
- 对已识别的4793种蛋白质进行了全面的注释.
- 分别在巴氏化和高压加工的果汁中发现了90和37个DAP.
- 加工果汁中丰富度增加的蛋白质与有机细胞组成部分有关,这表明细胞中断.
- 果糖二酸 (Fructose-bisphosphate aldolase) 被确定为一种加工标记物,原因是加工果汁中丰度和结构变化 (α-helix减少,随机线圈增加) 的减少.
结论:
- 蛋白质学提供了一种强大的方法来区分新鲜和加工的汁.
- 处理诱导的细胞破坏和蛋白质结构修饰是关键的差异化因素.
- 果糖二酸多酶作为汁加工的可靠标记物.
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