康米福拉 (Commiphora leptophloeos) 树叶和树皮提取物通过TLR4/NF-κB/Nrf2通路调节氧化炎症
Fernanda Barbosa Lopes1, Manoela Maciel Dos Santos Dias2, Silvânia Mól Pelinsari1
1Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil.
Journal of ethnopharmacology
|August 2, 2025
概括
康米福拉 (Commiphora leptophloeos) 树叶和树皮提取物通过调节关键分子通路,显示出显著的抗氧化和抗炎作用. 这些发现支持了这种植物在管理与氧化应激相关的炎症状况方面的传统用途.
科学领域:
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 传统上用于炎症状况的commiphora leptophloeos (伊姆布拉纳).
- 对其抗炎机制的分子理解有限.
研究的目的:
- 评估C. leptophloeos叶和树皮提取物在体外调节氧化应激诱导的炎症 (OxInflammation).
- 调查涉及的分子信号通路.
主要方法:
- 植物化学分析 (FIA-ESI-IT-MS/MS),抗氧化剂测定 (DPPH),抗炎性测定 (蛋白质变性,红细胞稳定,NO生产,COX活性).
- 对炎症标记物 (TLR4,NF-κB,BAX,COX-2,TNF-α,IL-6) 和抗氧化媒介物 (Nrf2,HO-1,HIF-1,IL-10) 的细胞活力 (MTT),触酶活性和基因表达分析 (RT-qPCR) 的分析.
主要成果:
- 叶子提取物富含黄酸盐;树皮提取物富含素.
- 这两种提取物都表现出强大的抗氧化和抗炎性质,减少氧化和促炎介质.
- Nrf2表达被上调,这表明抗氧化反应的激活.
结论:
- C. leptophloeos 提取物具有显著的抗氧化和抗炎性质,细胞毒性较低.
- 分子机制包括TLR4/NF-κB通路的减弱和Nrf2.2的上调.
- 提供了C. leptophloeos在治疗炎症疾病中的民族医学用途的科学验证.
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