来自得的狗中携带载体原生虫和细菌的高患病率使用向下一代测序方法确定
Ian Kirimi Daniel1, Hassan Hakimi1, Rafael A N Ramos1,2
1Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.
Scientific reports
|August 2, 2025
概括
在得的一项研究发现,88.7%的狗被至少一种载体传播病原体 (VBPs) 感染,大多数狗经历了并发感染. 高龄狗和南部查里巴吉米的狗有更高的风险.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子流行病学分子流行病学
- 狗的传染病 狗的传染病
背景情况:
- 关于影响撒哈拉以南非洲家养狗的载体传播病原体 (VBPs) 的数据有限.
- 了解VBP的患病率和相互作用对于该地区的狗健康管理至关重要.
研究的目的:
- 为了确定得犬群中VBPs的流行率.
- 为了确定与VBP感染相关的风险因素.
- 为了探索共感染狗之间的病原体相互作用.
主要方法:
- 针对性下一代测序 (tNGS) 试验被用于检测15个VBP.
- 在得,从1202只看似健康的狗身上采集了全血样.
- 使用多变量后勤回归和网络分析来评估风险因素和病原体相互作用.
主要成果:
- 观察到VBPs的高患病率,88.7%的狗感染至少一种病原体.
- 肝犬 (62.4%) 和 Mycoplasma haemocanis (59.2%) 是最常见的病原体.
- 同感染很常见 (62.9%),许多狗被三种或更多种VBP感染.
- 年龄较大和居住在南部的查里巴吉米被确定为危险因素.
- 网络分析表明,VBPs之间存在复杂的,促进性的关联.
结论:
- 本研究提供了查德犬群中VBP流行率和共感染的全面概述.
- 发现突出显著的病原体相互作用,并确定关键的风险因素.
- 建立了未来研究非洲犬类载体传播疾病的关键基准,特别是血液型菌质菌.
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