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hUC-MSCs和衍生的外体细胞通过调节雌激素信号通路来减弱DEX诱导的肌肉缩

Na Li^1,2,3, Xiaoqin Liu^4,2, Qiong Wang^4,2

¹Department of General Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Liaoning, People's Republic of China.

Stem cell research & therapy

|August 3, 2025

概括

人类带衍生中介质干细胞 (hUC-MSCs) 和它们的外体细胞 (MSC-Exos) 显示出对治疗肉症的前景. 这些疗法在临床前模型中改善了肌肉功能和质量,这表明在老年人群中具有临床应用的潜力.

科学领域:

再生医学是一种再生医学.
老年学是一门学科.
细胞生物学细胞生物学

背景情况:

标志着肌肉质量和力量损失的萨科佩尼亚是老龄化人口的主要公共卫生问题.
慢性炎症,不运动和内分泌问题加剧了萨尔科佩尼亚.
人类带衍生的介质干细胞 (hUC-MSCs) 和它们的外体细胞 (MSC-Exos) 显示出再生潜力,但它们对肉类的疗效尚未得到证实.

研究的目的:

评估hUC-MSCs和MSC-Exos在治疗肉症中的治疗潜力和安全性.
在肌肉缩模型中研究hUC-MSC和MSC-Exo治疗的潜在分子机制.

主要方法:

在雄性C57BL/6J小鼠中使用德克萨米他诱导的肌肉缩模型.
用hUC-MSCs,MSC-Exos或SNG162进行治疗,然后进行胃半腹肌分析.
在体外研究中,使用与hUC-MSCs或MSC-Exos共同培养的甲治疗的C2C12菌根管.
分析包括组织学,TUNEL染色,西部涂抹,RNA测序,qPCR,免疫光,EDU标记和ELISA.

主要成果:

在小鼠中,hUC-MSCs和MSC-Exos显著改善了肌肉力量,耐力和肌肉纤维大小.
治疗提高了关键肌肉再生标志物 (MyHC,Beclin-1,ERs,雌激素) 的调节,降低了缩/炎症标志物 (FOXO3,MURF1,TNF-α,IL-6) 的调节.
在促进肌细胞增殖和分化方面,MSC-Exos比hUC-MSC表现出优越的疗效,PI3K/AKT/mTOR和ERK1/2通路被确定为关键调解者.

结论:

hUC-MSCs和MSC-Exos代表了对萨科佩尼亚的新,安全和有效的治疗策略.
这些基于细胞的疗法为临床应用提供了有前途的途径,用于打击与年龄相关的肌肉衰退.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.自自是一种自的过程.德克萨米他是一种异构体是什么? 异构体是什么? 外构体是什么?人类带衍生中介细胞干细胞.肌肉缩肌肉缩肌肉缩萨尔科佩尼亚是什么意思萨尔科佩尼亚

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