小麦 WRKY 转录因子同源体在调节缺铁反应中的差调和相互作用
1Department of Biotechnology, National Agri-Food Biotechnology Institute, Mohali, India.
Plant biology (Stuttgart, Germany)
|August 3, 2025
概括
小麦 WRKY 转录因子 (TF) 在缺铁反应中起作用. 研究人员确定了18个上调的WRKY基因,并描述了它们的相互作用和W-box结合,提供了对铁平衡的见解.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 营养品条例 营养品条例 营养品条例
背景情况:
- 铁 (Fe) 对植物生长至关重要,需要复杂的调节网络来维持恒温.
- WRKY转录因子 (TFs) 是已知的调节者,但它们在缺铁反应中的作用在很大程度上尚未被探索.
研究的目的:
- 调查小麦 WRKY TF 网络在缺铁 (-Fe) 的情况下的功能.
- 为了确定参与调节铁吸收和同化基因的特定WRKYTFs.
主要方法:
- 定量RT-PCR分析WRKY基因表达在-Fe和循环赫西米德治疗下.
- 酵母-二混合和分裂-露西法酶测定 WRKY-WRKY 蛋白相互作用.
- 酵母-一混合和效应-报告测试以确定TF与W-box元素的结合.
主要成果:
- 在40个候选小麦WRKY (TaWRKY) 基因中,有18个在-Fe条件下升调.
- 特定的TaWRKY同质体表现出差异稳定性和相互作用能力.
- 在TaZIFL1促进体中,TaWRKY40-B4,TaWRKY18-A1和TaWRKY70-D1对W盒元素表现出不同的结合亲和力.
结论:
- 小麦 WRKY TFs参与调节对缺铁的反应.
- 同源性WRKYTFs表现出多样化的相互作用和结合特异性,有助于铁的平衡.
- 这些发现为了解六倍体小麦的Fe稳态网络提供了基础.
相关概念视频
Riboswitches
8.5K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.5K
Transcription
148.2K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
148.2K
Transcriptional Regulation: Riboswitches
114
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
114
Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
Regulation of the Unfolded Protein Response
2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K
Gene Regulation During Sporulation
78
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
78


