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细菌氧化还原传感器 OseR 通过 Cys 醇开关调节 ergothioneine 的吸收,增强氧化应激抵抗力和毒性
Xinchi Zhu1, Yifan Wu1, Huochun Yao1
1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210014, China; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing, 210014, China; WOAH Reference Lab for Swine Streptococcosis, Nanjing, 210014, China.
Redox biology
|August 3, 2025
概括
这项研究确定了OseR,这是一种新型细菌抑制剂,可以调节对氧化应激反应的ergothioneine (ET) 运输. OseR控制ET吸收和细菌毒性,为微生物适应提供了洞察力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 欧戈氨酸 (ET) 是一种强大的抗氧化剂,由少数微生物合成,但由许多微生物获得.
- 存在用于ET运输 (EtUV) 的微生物系统,但其监管仍然不清楚.
- 葡萄球菌 (Streptococcus suis) 是猪和人类的重要病原体.
研究的目的:
- 确定和描述Streptococcus suis.中ET传输系统 (EtUV) 的监管机制.
- 阐明MarR家族抑制剂OseR在调节ET吸收和细菌对氧化应激反应中的作用.
主要方法:
- 在Streptococcus suis.中OseR抑制剂的识别和表征.
- 在氧化应激条件下对OseR与EtUV操作子促进物的相互作用进行分析.
- 评估oseR和etU突变对细菌氧化应激耐受性,巨细胞存活率和小鼠毒性的影响.
主要成果:
- OseR充当基于醇的开关,通过Cys35.5感知氧化应激.
- 氧化应激触发了OseR与EtUV促进体的解离,激活了ET运输.
- OseR还调节其他LMW硫醇转运体和氧化应激反应基因.
- oseR和etU突变显著降低了细菌的毒性和氧化应激耐受性.
结论:
- OseR是Streptococcus suis中ET传输的关键调节者,由一个醇开关机制控制.
- 通过OseR调节ET吸收,对于细菌适应和宿主环境中的毒性至关重要.
- 这些发现表明,OseR在跨物种的细菌适应中具有保留的调节作用.
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