硫甲在沉积物中的生物降解是由慢性暴露度驱动的
Hélène Rogue1, Cécile Miège1, Chloé Bonnineau2
1INRAE, UR RiverLy, Villeurbanne 69625, France.
Ecotoxicology and environmental safety
|August 3, 2025
概括
高度的硫甲 (SMZ) 显著提高了河流沉积物中的自然微生物群落的生物降解能力. 这种适应涉及特定的基因,并改变微生物组成,而不会增加社区对抗生素的整体耐受性.
科学领域:
- 环境微生物学环境微生物学
- 环境化学环境化学
- 生态毒理学 生态毒理学
背景情况:
- 硫胺抗生素,如硫甲 (SMZ),可以在河流沉积物中持续存在.
- 没有充分了解SMZ暴露值对硫胺降解微生物的发展的影响.
研究的目的:
- 调查硫胺的微生物生物降解能力是否被显著刺激超过特定的SMZ暴露值.
- 确定关键基因和与SMZ生物降解相关的微生物社区转移.
主要方法:
- 在一项为期47天的微观宇宙研究中,自然沉积物群体被暴露在6种SMZ度 (C0-C5,最大10mg.kg-1DW) 中.
- 用C-SMZ矿化动力学来评估生物降解.
- 分析了基因丰度 (sadA) 和微生物社区结构 (16S rRNA测序).
- 对β-葡萄糖酶活性进行的短期毒性测试评估了社区的耐受性.
主要成果:
- 显著的SMZ生物降解 (50-62%矿化) 仅发生在两个最高度 (C4,C5) 时.
- 在C4和C5中,sadA基因的相对丰度增加,表明它在SMZ生物降解中的作用.
- 微生物社区的结构,多样性和组成发生了变化,蛋白质细菌增加,蓝藻细菌/细菌菌减少.
- 通过β-葡萄糖酶活性测量SMZ的社区耐受性不受增加的生物降解潜力的影响.
结论:
- 对于SMZ度存在一个明显的值,该度可显著刺激河流沉积物中的生物降解.
- sadA基因可能是SMZ生物降解的关键功能基因.
- SMZ适应改变了微生物群落的动态,但并没有增加对抗生素的耐受性.
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