乳酸细菌中一种新型的自身聚合,由Streptococcus thermophilus CC40-4S的新AggS聚合因子促进
Darya Tsibulskaya1, Marinela Šokarda Slavić2, Amarela Terzić-Vidojević3
1Institute of Virology, Vaccines and Sera "Torlak", Vojvode Stepe 458, Belgrade, Republic of Serbia.
International journal of biological macromolecules
|August 3, 2025
概括
研究人员在Streptococcus thermophilus中发现了一种新的自聚基因,即aggS,它是一种有益的益生菌. 这一发现扩大了我们对乳酸细菌中的聚合促进因子 (APF) 的理解.
科学领域:
- 微生物学 微生物学
- 益生菌 益生菌 益生菌
- 食品科学 食品科学 食品科学
背景情况:
- 自聚是乳酸细菌中有益的益生菌特征,增强它们的食品和医疗应用的特性.
- 雪花形成的原结合聚合因子 (SFCBAFs) 是已知的乳酸细菌中的大型聚合促进因子 (APFs).
- 研究了一种在Streptococcus thermophilus CC40-4S中的新型自身聚合机制.
研究的目的:
- 鉴定和描述Streptococcus thermophilus中的一个新的自聚类表型的遗传基础.
- 确定在S. thermophilus. 自动聚合中的假定aggS基因的功能.
- 为了将新发现的聚合因子与已知的SFCBAF型APF进行比较.
主要方法:
- 全基因组测序的自我聚合阳性S.热 CC40-4S.的.
- 生物信息分析用于预测aggS基因.
- 通过同类重组和异质表达在Lactococcus lactis中的基因破坏.
主要成果:
- 在S. thermophilus CC40-4S.中发现了一种假定的自聚基因,即aggS.
- 对aggS的破坏取消了自身聚合的表型.
- 在Lactococcus lactis中AggS的异质表达诱导了自聚,证实了它的作用.
- AggS是一种大型蛋白质 (237 kDa),具有重复区域,但缺乏原结合域,形成小聚合物.
结论:
- 这项研究报告了第一个编码Streptococcus thermophilus聚合促进因子的基因.
- 鉴定到的AggS蛋白代表了乳酸细菌中一种新型的自身聚合因子.
- 这一发现为设计S. thermophilus中的益生菌特性提供了新的可能性.
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