高准确度的组织超分辨率成像是通过共聚焦旋盘图像扫描显微镜实现的
Qianxi Liang1, Wei Ren1, Boya Jin1
1Department of Biomedical Engineering, National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing, China.
Light, science & applications
|August 3, 2025
概括
我们开发了一种新的深层组织超高分辨率显微镜,confocal2旋转磁盘图像扫描显微镜 (C2SD-ISM),以克服背景干扰并改善生物研究的成像深度和真实性.
科学领域:
- 生物光子学和先进的成像技术.
- 显微镜和超高分辨率成像.
- 细胞和组织成像.
背景情况:
- 深层组织超高分辨率成像受到背景噪声的限制,降低了透深度和图像质量.
- 现有的方法在更深处与散射和光学偏差作斗争.
- 在厚厚的生物样本中高准确度可视化亚细胞结构仍然是一个挑战.
研究的目的:
- 引入一种新的成像系统,confocal2旋转磁盘图像扫描显微镜 (C2SD-ISM),用于增强深层组织超高分辨率成像.
- 克服背景干扰的局限性,提高生物样本的成像准确度.
- 为了实现比以前更深的高分辨率成像.
主要方法:
- 一个旋盘 (SD) 协焦显微镜与数字微镜装置 (DMD) 的集成,用于稀疏的多焦照明.
- 实现动态针孔阵列像素重新分配 (DPA-PR) 算法用于图像扫描显微镜 (ISM) 超分辨率重建.
- 双对焦配置可增强分辨率并减轻背景散射.
主要成果:
- 实现了高达180微米的成像深度,随着深度的增加保持了强度分布.
- 获得了144nm的横向分辨率和351nm的轴向分辨率.
- 证明了高保真度,原始对焦和重建图像之间有92%的线性相关性,纠正了误差和斯托克斯移位.
结论:
- C2SD-ISM系统有效地减轻了背景散射,并提高了深层组织成像的分辨率.
- 该系统提供高保真超分辨率成像,具有改进的深度透和偏差校正.
- 它的多功能性和可扩展性,包括与结构化照明显微镜的兼容性,使其成为生物成像的宝贵工具.
更多相关视频
12:44Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
Published on: September 29, 2014
20.1K
10:19Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
Published on: January 21, 2019
6.6K
相关概念视频
01:37Super-resolution Fluorescence Microscopy
7.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.7K
01:16Confocal Fluorescence Microscopy
14.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
14.3K
01:28Three-Dimensional Microscopy in Microbiology
295
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
295