贝纳拉西尔-M及其两个土壤代谢物的C14标记合成
Entela Sinani1, Stefano Garau1, Francesca Rizzo1
1Metabolism & Environmental Fate Department, Gowan Novara, Novara, Italy.
Journal of labelled compounds & radiopharmaceuticals
|August 4, 2025
概括
杀菌剂Benalaxyl-M及其代谢物的放射合成是通过使用标记为中介的新型物来实现的. 这种方法优化了放射性前体的使用,以便在土壤研究中准确跟踪.
科学领域:
- 农业化学 农业化学
- 有机合成 有机合成
- 放射化学 放射化学是指辐射化学.
背景情况:
- 虫病对作物生产构成重大威胁.
- 有效的杀菌剂对于对虫虫病的控制至关重要.
- 放射性标记化合物对于研究杀菌剂在土壤中的命运和有效性至关重要.
研究的目的:
- 为系统性真菌杀菌剂Benalaxyl-M及其土壤代谢产物开发放射合成方法.
- 为了利用一个通用的通用中间体,[-U-14C]N-{2,6-dimethylphenyl) -D-alanine甲基,用于合成.
- 优化标记前体的使用,以实现高效的放射性标记.
主要方法:
- 贝纳拉西尔-M和两个土壤代谢物的放射合成.
- 使用[-U-14C]N-(2,6-二甲基) -D-氨酸甲基作为一个关键的中间体.
- 采用 (S) - 乳酸甲基的三酸,以提高反应性和反体纯度.
主要成果:
- 成功放射合成贝纳拉克西尔-M及其相关的土壤代谢产物.
- 通过使用三叶酸中间体,优化了[phenyl-U-14C]2,6-dimethylaniline的使用.
- 在室温下达到具有高反体纯度和提高产量的反应.
结论:
- 已经建立了一个强大的放射合成路径,用于Benalaxyl-M及其代谢物.
- 开发的方法可以有效地跟踪和研究土壤中的杀菌剂行为.
- 使用三叶酸中间体在放射性标记合成中提供了产量和酶选择性方面的优势.
相关概念视频
01:11Nucleophilic Aromatic Substitution: Elimination–Addition
Simple aryl halides do not react with nucleophiles. However, nucleophilic aromatic substitutions can be forced under certain conditions, such as high temperatures or strong bases. The mechanism of substitution under such conditions involves the highly unstable and reactive benzyne intermediate. Benzyne contains equivalent carbon centers at both ends of the triple bond, each of which is equally susceptible to nucleophilic attack. This 50–50 distribution of products is confirmed through isotopic...
01:37NMR Spectroscopy of Benzene Derivatives
Simple unsubstituted benzene has six aromatic protons, all chemically equivalent. Therefore, benzene exhibits only a singlet peak at δ 7.3 ppm in the 1H NMR spectrum. The observed shift is far downfield because the aromatic ring current strongly deshields the protons. Any substitution on the benzene ring makes the aromatic protons nonequivalent, and the protons split each other. The peak is, therefore, no longer a singlet and the splitting pattern and their associated coupling constants depend...
01:19Phase I Reactions: Oxidation of Aliphatic and Aromatic Carbon-Containing Systems
Phase I biotransformation reactions are integral to drug metabolism, predominantly involving oxidative, reductive, and hydrolytic transformations. Chief among these are oxidative reactions, which enhance the hydrophilicity of xenobiotics and introduce polar functional groups to facilitate their elimination from the body.
Oxidation reactions are fundamental in aromatic carbon-containing systems. An example is the hydroxylation of phenobarbital, a process that transforms it into...
Oxidation reactions are fundamental in aromatic carbon-containing systems. An example is the hydroxylation of phenobarbital, a process that transforms it into...