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建立和评估HepG2细胞胰岛素抵抗模型

Zixuan Meng¹, Yuehua Han¹, Linda Ruan¹

¹School of Pharmacy, Bengbu Medical University, Bengbu, People's Republic of China.

Diabetes, metabolic syndrome and obesity : targets and therapy

|August 4, 2025

概括

在HepG2细胞中建立可靠的胰岛素抵抗模型至关重要. 棕酸治疗提供了一种简单,稳定的方法来诱导胰岛素抵抗,其性能优于其他测试方法.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
代谢研究研究代谢研究

背景情况:

胰岛素抵抗是代谢障碍的一个关键因素.
开发可靠的细胞模型对于研究胰岛素耐药性至关重要.
肝肝G2细胞是肝脏研究中广泛使用的模型.

研究的目的:

建立和评估诱导HepG2细胞胰岛素抵抗的不同方法.
为了比较葡萄糖胺,高葡萄糖与高胰岛素以及棕酸在制造胰岛素抵抗模型中的有效性.
为未来的胰岛素抵抗研究确定最可靠的方法.

主要方法:

用葡萄糖胺治疗HepG2细胞,用高胰岛素治疗高葡萄糖,或用棕酸诱导胰岛素抵抗.
评估葡萄糖摄入量和消耗量.
评估与葡萄糖代谢相关的mRNA表达和p-AKT/AKT蛋白水平.
进行RNA测序以分析转录原子差异.

主要成果:

棕酸和葡萄糖胺降低了葡萄糖消耗能力.
棕酸降低了葡萄糖吸收能力.
每种方法对G6酶,PC和PCK1的基因表达发生了不同的改变.
RNA-seq揭示了三种诱导方法之间的转录组的显著差异.

结论:

用0.2mM棕酸治疗24小时是一种简单而稳定的方法,可以在HepG2细胞中诱导胰岛素抵抗.
这种方法为进一步的胰岛素抵抗研究提供了可靠的模型.
独特的分子形状凸显了选择适当的诱导方法的重要性.

关键词:

在HepG2细胞中.胰岛素耐药性模型棕酸是一种棕酸.

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