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Updated: Sep 13, 2025

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
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Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

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鉴定阿斯巴提尔-tRNA合成酶与小麦胚芽eIF2共同净化

Ophelia Papoulas1, Edward M Marcotte1, Karen S Browning1

  • 1Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712 USA.

microPublication biology
|August 4, 2025
PubMed
概括

研究人员确定了一种~61kDa的蛋白质与小麦胚芽中的真核转化启动因子2 (eIF2) 共同净化. 这种蛋白质被确定为阿斯巴提尔-tRNA合成酶,可能将翻译启动与细胞应激反应联系起来.

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tRNA Activation02:26

tRNA Activation

Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
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