Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Abnormal Proliferation02:23

Abnormal Proliferation

4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Negative Regulator Molecules01:23

Negative Regulator Molecules

36.0K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
36.0K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.3K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.4K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.4K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

7.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
7.2K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

4.9K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The Target ALS Global Natural History Study: Cross-platform proteomics to accelerate biofluid biomarker and drug target discovery in amyotrophic lateral sclerosis.

medRxiv : the preprint server for health sciences·2026
Same author

Chemoselective Halogenation of Premarineosin A for Next-Generation Antimalarial Development.

bioRxiv : the preprint server for biology·2026
Same author

Postmortem Evidence of Lymphatic Vessels Located at the Boundary of Central and Peripheral Nervous Systems in the Cervical Spine.

Cellular and molecular neurobiology·2026
Same author

Correction: Spatial resolution of the metastatic osteosarcoma tumor microenvironment using immunolabeling across murine, canine and human lung.

Journal of translational medicine·2026
Same author

AI-powered and manual assessment of tumor-infiltrating lymphocytes in early HER2-positive breast cancer in NSABP B-41.

NPJ breast cancer·2026
Same author

Nicotinamide Riboside (NR) Supplementation Exerts Neuroprotective Effects in db/db Mouse Model of Type 2 Diabetes.

Journal of molecular neuroscience : MN·2026

相关实验视频

Updated: Sep 12, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.6K

一个定量高通量屏幕识别了可以抑制p53异型Δ133p53α并抑制细胞衰老的化合物.

Delphine Lissa1, Sebastien M Joruiz1, Patricia K Dranchak2

  • 1Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, United States.

ACS pharmacology & translational science
|August 4, 2025
PubMed
概括

研究人员开发了一种新的测定方法,以寻找增强D133p53α的化合物,这种蛋白与细胞衰老作斗争. 两种化合物,AZD1981和切拉斯特,被确定,可能为与衰老相关的疾病提供新的治疗方法.

关键词:
AZD1981 年的AZD.星球细胞是星球细胞.塞拉斯特罗尔 (celastrol) 是一种化学图书馆的化学图书馆p53 的同位体形式.这是一个定量高通量屏幕.

更多相关视频

Induction and Validation of Cellular Senescence in Primary Human Cells
08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

17.2K
Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

33.8K

相关实验视频

Last Updated: Sep 12, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.6K
Induction and Validation of Cellular Senescence in Primary Human Cells
08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

17.2K
Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

33.8K

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • Δ133p53α是一种人类/灵长类动物特有的p53异型,可以抑制细胞衰老和相关的分泌表型 (SASP).
  • 伴奏辅助选择性自 (CASA) 是通过降解调节 Δ133p53α 蛋白水平的关键机制.
  • 细胞衰老与衰老和与年龄相关的疾病有关.

研究的目的:

  • 开发和实施定量高通量选 (qHTS) 试验,以识别可上调 Δ133p53α 蛋白水平的化合物.
  • 通过向Δ133p53α调节,发现针对衰老和衰老相关疾病的新型治疗剂.

主要方法:

  • 开发了一种使用光标记 Δ133p53α 的基于细胞的新型 qHTS 试验.
  • 超过1万种小分子化合物被选,以确定Δ133p53α的调节剂.
  • 候选化合物被证实具有提高内源 Δ133p53α 调节和减少衰老标志物的能力.

主要成果:

  • 通过qHTS测试,成功地分析了超过1万种化合物.
  • 两种化合物,AZD1981和,被确定为人类星球细胞和纤维细胞中Δ133p53α蛋白水平的强烈诱导剂.
  • 塞拉斯托尔的作用与CASA介导的调节一致,涉及热冲击蛋白70 (HSP70) 陪伴者.
  • 对Δ133p53α的升调导致细胞衰老和SASP因子分泌的减少.

结论:

  • 开发的qHTS试验有效地识别了调节Δ133p53α的化合物.
  • AZD1981和赛拉斯特显示出治疗衰老和衰老相关疾病的治疗潜力.
  • 针对 Δ133p53α 稳定提供了一个有前途的战略,用于开发新的抗衰老疗法.