用于生物传感应用的Zwitterionic防聚合物涂层对热水合的基线稳定性
Soren M Smail1, Paul E Laibinis1,2, Sharon M Weiss1,3
1Interdisciplinary Materials Science Graduate Program, Vanderbilt University, Nashville, Tennessee 37235, United States.
ACS omega
|August 4, 2025
概括
多孔生物传感器通过使用一种新的表面改性来实现更好的稳定性和更低的检测极限. 这种防涂层可以防止复杂的生物样本的水解和非特异性结合.
科学领域:
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
背景情况:
- 基于多孔 (PSi) 的生物传感器为快速诊断提供了潜力,但其基线稳定性不佳.
- PSi的水解和不需要的物种的生物污染限制了临床样本的检测灵敏度.
- 解决这些挑战对于推进PSI生物传感器临床适用性至关重要.
研究的目的:
- 为多孔生物传感器开发一种稳定且防的表面修饰.
- 改进基于PSI的诊断平台的检测极限.
- 为了创建一个通用的表面化学,用于整合特定的捕获剂.
主要方法:
- 用PSi与乙烯基化 (VBC) 进行热化,以创建Si-C键和基化物终结.
- 表面启动的原子转移激素聚合 (siATRP) 来移植聚硫乙烯甲基酸盐 (SBMA) 以获得抗污染性质.
- 光学反射率和ATR-FTIR测量以评估PBS和人血清中的水解和非特异性吸附.
主要成果:
- PSi-VBC-polySBMA表面显示出水解稳定性,并显著减少了非特异性结合.
- 接种的polySBMA层提供了有效的抗物特性,通过在生物流体中吸附的最小程度得到证实.
- 表面修改策略与各种化学组相兼容,使多功能功能化成为可能.
结论:
- 开发的水化和siATRP表面化学有效地减轻了PSI水解和生物污染.
- 这种方法提高了多孔生物传感器的基线稳定性.
- 该战略显示,在复杂的生物样本中实现临床相关的检测极限具有前景.
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