调节HRAS1 I-动机DNA促进区的饮食植物黄酸甲二醇
Sagar Bag1, Souvik Ghosal2, Mangal Deep Burman1
1Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
ACS omega
|August 4, 2025
概括
凯姆菲罗尔 (KAE) 选择性地结合和稳定i-motif (IM) DNA,这是一种与癌症有关的结构. 这种相互作用显示出开发新的抗癌药物和瘤细胞的诊断工具的潜力.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 药用化学 医学化学
背景情况:
- 非正规DNA结构,如i-motif (IM) DNA,参与细胞过程和癌症等疾病.
- IM DNA 在基因调节中起到分子开关的作用,特别是在致癌促进体和端粒区域.
- 针对IM DNA是药物开发的一个有前途的战略.
研究的目的:
- 为了研究甲醇 (KAE) 和多个IMDNA配置之间的相互作用,包括HRAS1 IM.
- 为了确定KAE和IMDNA与双重DNA之间的结合亲和力和相互作用方式.
- 探索KAE作为癌症治疗剂和诊断工具的潜力.
主要方法:
- 吸收光谱法对紫外线的吸收光谱.
- 光光谱学是一种光谱学.
- 化的温度分析 (Tm)
- 时间解决的光衰变.
- 循环二重化 (CD) 光谱学 循环二重化 (CD) 光谱学
- 热力学分析热力学分析
主要成果:
- 在与HRAS1 IM DNA结合时,KAE表现出高染色效应和光谱转移.
- KAE显著增加了HRAS1 IM DNA的发射强度和化温度,表明了强大的稳定性.
- KAE优先结合HRAS1 IM DNA而不是双重DNA,具有更高的结合常数.
- 谱学数据表明一个强大的堆叠相互作用模式.
- 热力学参数表明一种自发的,由力驱动的结合过程.
结论:
- 凯姆菲罗尔 (KAE) 选择性地准并稳定HRAS1的i-motif (IM) DNA.
- 通过调节转录调节,KAE显示出作为抗癌药物候选药物的潜力.
- 凯埃的光特性可能会使创新的光学应用用于检测和量化瘤细胞中的IM DNA.
相关概念视频
The Eukaryotic Promoter Region
16.6K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
16.6K
Co-activators and Co-repressors
7.5K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.5K
MAPK Signaling Cascades
6.1K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
6.1K
Master Transcription Regulators
7.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.0K
Cell Specific Gene Expression
13.9K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.9K
RNA Polymerase II Accessory Proteins
9.5K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.5K


