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Updated: May 3, 2026

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
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NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

Published on: June 30, 2019

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一个有效的策略,可以快速确定腺脱氨酶和纯核酸酸化酶的动力学,并选它们的抑制剂

Pingyi Zheng1, Xinru Wang1, Ruijun Tang1

  • 1Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Faculty of Medicine, Tianjin University, China.

International journal of biological macromolecules
|August 4, 2025
PubMed
概括

这项研究引入了一种新的毛细管电泳法,用于快速分析氨酸脱氨酶 (ADA) 和纯核酸酸酶 (PNP) 的酶动力学. 该技术有效选酶抑制剂,为传统离线方法提供可靠的替代方案.

关键词:
亚丁氨酸脱氨酶的使用显而易见的米开利斯常数毛细管电泳可以通过毛细管电泳进行.抑制常数是一种阻碍常数.抑制剂是一种抑制剂.酶的动力常数是酶的动力常数.纯氨酸核酸酸化酶是一种纯氨酸核酸酸化酶.

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