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β-catenin 缺乏对脂肪组织生理学的影响

Romina M Uranga¹, Akira Nishii², Jessica N Maung²

¹University of Michigan Medical School, Department of Molecular & Integrative Physiology, Ann Arbor, MI, USA; Instituto de Investigaciones Bioquímicas de Bahía Blanca, Universidad Nacional del Sur-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bahía Blanca, Argentina; New College of Florida, Natural Sciences Division, Sarasota, FL, USA.

Molecular metabolism

|August 4, 2025

概括

在男性白色脂肪组织中破坏Wnt/β-catenin信号传递会增加脂肪和胰岛素抵抗. 其他细胞中的补偿Wnt信号有助于维持平衡,突出显示其在脂肪组织中的关键作用.

科学领域:

内分泌学和新陈代谢学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

规范的Wnt/β-catenin信号传递对白色脂肪组织 (WAT) 发育和生理学至关重要.
这种途径的失调会影响脂肪和代谢平衡.
了解脂肪细胞Wnt/β-catenin破坏如何影响基因表达和细胞间交叉是必不可少的.

研究的目的:

研究脂肪组织在脂肪细胞中缺少β-catenin时维持恒常的机制.
探索脂肪细胞特异性β-catenin缺陷对WAT内的基因表达和细胞-细胞通信的影响.

主要方法:

脂肪细胞特异性β-catenin淘汰赛小鼠 (Ctnnb1^AdKO) 的代谢表型.
大量和单细胞RNA测序 (scRNA-seq) 用于分析转录组变化.
流细胞计和分泌的细胞外囊泡的隔离,以进行全面的分析.

主要成果:

雄性Ctnnb1^AdKO小鼠表现出增加的脂肪和胰岛素抵抗,与雌性不同.
在脂肪细胞中抑制脂肪酸代谢与增强的免疫细胞线粒体代谢形成对比.
β-catenin缺乏改变了包括免疫细胞在内的肌体血管细胞的转录组,在内皮细胞和细胞外囊中发现了补偿Wnt信号.

结论:

脂肪细胞Wnt信号传递对于调节肌体血管细胞和脂肪细胞中的脂质和线粒体代谢至关重要.
细胞间Wnt信号网络显示出补偿能力,可以维持脂肪组织的平衡.
Wnt/β-catenin信号传递对于理解脂肪组织生理学和病理生理学至关重要.

关键词:

脂肪细胞脂肪细胞肥胖的是一种脂肪.细胞外囊泡中的细胞外囊泡.巨细胞是一个巨细胞.线粒体中的线粒体.有反应性氧物种的反应性氧物种.没有信号发送.这是一种β-catenin.

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