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背脊根结节中的动态分子景观用于通过组织工程神经移植促进的外周神经再生

Hui Zhu¹, Siyuan Xu², Yiting Wang²

¹Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong, JS 226001, PR China.

Journal of advanced research

|August 4, 2025

概括

组织工程神经移植 (TENGs) 使用皮肤衍生前体施万氏状细胞 (SKP-SCs) 促进高级神经再生. 这项研究揭示了分子机制,确定了促进神经元存活和增长的副因子,以修复外围神经.

科学领域:

生物材料科学生物材料科学
神经科学是一个神经科学.
再生医学是一种再生医学.

背景情况:

由于神经系统再生有限,外围神经损伤带来了重大挑战.
组织工程神经移植 (TENGs) 使用皮肤衍生前体施万氏状细胞 (SKP-SCs) 和丝纤维蛋白-基托桑支架显示出有前途.
对于TENG优越于自体移植和支架的基本机制需要阐明.

研究的目的:

调查SKP-SCs-TENGs观察到的增强神经再生背后的分子机制.
确定TENGs在外围神经修复中的有效性负责的特定因素和途径.

主要方法:

关于大鼠背部根 (DRG) 的综合转录基因分析.
对SKP-SCs条件介质进行LC-MS/MS分析.
共同培养的DRG神经元的RNA测序.
生物信息分析包括因果网络和蛋白质与蛋白质相互作用网络分析.

主要成果:

转录和实时PCR数据证实了神经再生的增强.
生物信息学确定了候选分泌的蛋白质 (神经营养/再生因素) 和相关的信号通路.
共同培养实验证明了SKP-SCs的膜效应,促进DRG神经元的存活和生长.
对DRG神经元的RNA测序突出了调制的分子通路.

结论:

结合生物材料,细胞疗法和奥米克技术的综合方法对外围神经修复有效.
这项研究为推进基于TENG的疗法进行临床应用提供了临床前基础.
了解SKP-SCs-TENG疗效的分子基础对于优化神经再生策略至关重要.

关键词:

背部根结腺细胞.基因组丰富分析分析周围神经的再生和再生在SKP-SCs中使用.由皮肤获得的前体.组织工程是组织工程.

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