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虹膜调节牛细胞中的IFNT信号

Ana Paula da Silva¹, Karine de Vargas Aires¹, Suzana Rossato Feltrin¹

¹Graduate Program of Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.

Molecular reproduction and development

|August 5, 2025

概括

纤维素3型域含有4 (FNDC4) 和纤维素3型域含有5 (FNDC5),是一种虹膜素的前体,是牛生育的关键. 虹膜素增强干细胞中干激发基因,可能改善胚胎信号传递.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
细胞生理学细胞生理学

背景情况:

阿迪波金调节能量状态和生殖,影响卵巢生理学.
纤维素第III类域含有 (FNDC) 家庭成员,FNDC4和FNDC5 (素前体),对于生育至关重要.
了解FNDC4,FNDC5及其在牛体白色体 (CL) 中的受体对于生殖健康至关重要.

研究的目的:

在牛的CL寿命阶段调查FNDC4,FNDC5和受体 (ITGB1,ITGAV,ADGRF5) 的表达.
评估伊丽素对牛质细胞中干扰素 (IFNT) 介导的信号传递的影响.
确定伊丽素在牛生育中的作用以及潜在的补偿机制.

主要方法:

定量实时PCR (qPCR) 用于在不同阶段评估FNDC4,FNDC5和牛CL受体的mRNA表达.
在体外用素对牛质细胞进行治疗,以分析对IFNT刺激基因 (ISG),类固醇酶,活力,增殖和亡的影响.
在受精后的第18天,怀孕和非怀孕的母牛之间的基因表达的比较.

主要成果:

与早期I相比,在较晚的CL发育阶段 (早期II,中期,晚期) 的FNDC4和FNDC5mRNA丰度显著更高.
在较晚的CL阶段,ITGB1和ADGRF5的表达更大,而ITGAV没有显著差异.
在第18天,非孕母牛的CL中受体mRNA表达率较高.
在体外,虹膜素治疗显著增加了白细胞中的ISG (MX1,MX2),增强了IFNT信号传递,但没有影响类固醇生成或细胞活力.

结论:

在整个牛的CL发育过程中,FNDC4和FNDC5都被表达,在后期阶段的水平更高.
虹膜素增强了牛细胞中的ISG表达,这表明它在增强IFNT信号传递中的作用.
虹膜素对CL功能的影响可能是间接的,可能在负能量平衡期间作为补偿机制来改善胚胎信号传递.

关键词:

在IFNT信号传输中.亚迪波基因 (Adipokine) 是一种可靠的药物.黄体体 (Corpus luteum) 是一个体.虹膜是一种虹膜.

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