在Neoseiulus barkeri嗅觉感知中,作为气味载体的C2型Niemann-Pick蛋白的功能性表征
Haozhuo Yang1,2, Tiandi Niu1,2, Rongjiang Ma1
1Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, College of Plant Protection, Southwest University, Chongqing, China.
Insect science
|August 5, 2025
概括
掠食性虫中的Niemann-Pick蛋白类型C2 (NPC2) 作为植物挥发物的气味载体,对于食和交配行为至关重要. 这项研究揭示了NPC2.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 化学生态化学生态学
背景情况:
- 节肢动物依靠嗅觉线索来实现重要的生命功能,如寻找食物和繁殖.
- 尼曼-皮克蛋白类型C2 (NPC2) 是一种潜在的气味载体,但其在捕食虫嗅觉中的作用尚未完全理解.
研究的目的:
- 在掠食性虫Neoseiulus barkeri中描述NbNPC2-2和NbNPC2-3的嗅觉感知功能.
- 阐明了虫对草食动物诱导的植物挥发物 (HIPV) 的嗅觉反应背后的分子机制.
主要方法:
- 对NbNPC2-2和NbNPC2-3对甲基酸盐 (MeSA) 的反应进行基因表达分析.
- 通过RNA干扰 (RNAi) 来抑制NbNPC2-2和NbNPC2-3的表达.
- 在MeSA处理下测量植物偏好和爬行速度的行为测试.
- 结合亲和分析和分子对接,以评估NPC2蛋白和HIPV之间的相互作用.
主要成果:
- 作为对MeSA的反应,NbNPC2-2和NbNPC2-3的调节显著上升.
- 两种NbNPC2基因的淘汰会影响虫对受感染植物的偏好,并降低爬行速度.
- NPC2-2与β-离子子,MeSA和奥基结合,而NPC2-3与4-乙基甲结合.
- 分子对接证实了NPC2蛋白和HIPV之间的键相互作用.
结论:
- 在Neoseiulus barkeri中,NbNPC2-2和NbNPC2-3作为气味载体起作用.
- 这些蛋白质是虫对HIPV的嗅觉感知不可或缺的组成部分,影响食行为.
- 这些发现有助于理解植物菌体嗅觉的分子基础.
更多相关视频
08:42Whole Mount Labeling of Cilia in the Main Olfactory System of Mice
Published on: December 27, 2014
11.4K
10:16Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
Published on: July 13, 2015
26.8K
相关概念视频
Olfactory Receptors: Location and Structure
9.6K
The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
9.6K
Physiology of Smell and Olfactory Pathway
9.5K
Humans detect odors with the help of specialized cells located in the upper part of the nasal cavity, called olfactory receptor neurons (ORNs). ORNs possess hair-like structures called cilia, which are receptive to sensations from the inhaled air. When an odorant molecule binds to a specific receptor on the cell of the cilia, it leads to a series of events that ultimately cause the ORN to send electrical signals to the olfactory bulb in the brain through the olfactory nerves.
The olfactory...
The olfactory...
9.5K
Olfaction
45.2K
The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...
The olfactory receptors are embedded in the cilia of the...
45.2K
G-Protein Gated Ion Channels
4.8K
GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
Sensory...
4.8K
Tactile and Chemical Senses
360
Tactile senses encompass touch, temperature, and pain, each mediated by specific receptors. Touch receptors detect mechanical energy or pressure against the skin. Sensory fibers from these receptors enter the spinal cord and relay information to the brain stem. Here, most fibers cross over to the opposite side of the brain. The touch information then moves to the thalamus, which projects a map of the body's surface onto the somatosensory areas of the parietal lobes in the cerebral cortex.
360
Gustation
48.9K
Gustation is a chemical sense that, along with olfaction (smell), contributes to our perception of taste. It starts with the activation of receptors by chemical compounds (tastants) dissolved in the saliva. The saliva and filiform papillae on the tongue distribute the tastants and increase their exposure to the taste receptors.
48.9K
