Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

171
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
171
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

278
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
278

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The AadR-FixK hierarchy coordinates iron-responsive metabolism via Fur-family regulators in <i>Rhodopseudomonas palustris</i> TIE-1.

bioRxiv : the preprint server for biology·2026
Same author

Reductive evolution and genomic reduction in <i>Rhodopseudomonas</i>.

Applied and environmental microbiology·2025
Same author

Reply to "Genome of <i>Rhodovulum iodosum</i>, a marine photoferrotroph".

Microbiology resource announcements·2025
Same author

Correction for Gupta et al., "Draft Genome Sequence of a Marine Photoferrotrophic Bacterium, <i>Rhodovulum iodosum</i> DSM 12328<sup>T</sup>".

Microbiology resource announcements·2025
Same author

Purple non-sulfur bacteria for biotechnological applications.

Journal of industrial microbiology & biotechnology·2024
Same author

Overexpression of RuBisCO form I and II genes in <i>Rhodopseudomonas palustris</i> TIE-1 augments polyhydroxyalkanoate production heterotrophically and autotrophically.

Applied and environmental microbiology·2024

相关实验视频

Updated: Sep 12, 2025

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K

最先进的方法量化微生物多基酸.

Eric M Conners1, Arpita Bose1

  • 1Department of Biology, Washington University in St. Louis, St. Louis, Missouri, USA.

Applied and environmental microbiology
|August 5, 2025
PubMed
概括

量化聚基酸盐 (PHAs) 需要仔细选择方法. 本综述比较了PHA测量流行的和新兴的技术,帮助研究人员选择最适合他们的需求的方法.

科学领域:

  • 生物技术是生物技术.
  • 聚合物科学 聚合物科学
  • 微生物学 微生物学

背景情况:

  • 聚酸酸 (PHAs) 是微生物合成的可生物降解聚合物,为生物塑料提供可持续的替代品.
  • 准确量化PHA生产对于优化微生物菌株和生物过程至关重要.
  • 目前用于PHA量化的方法在准确度,灵敏度和资源要求方面各不相同.

研究的目的:

  • 为了提供一个全面的概述的流行和新兴的实验室方法量化聚酸酸盐 (PHAs).
  • 根据后勤和技术考虑,指导研究人员选择适当的PHA量化技术.
  • 突出研究缺口,并提出科学界对PHA测量的最佳实践.

主要方法:

  • 对PHA定量化的既定和新型实验室技术的审查和比较.
  • 分析设备,程序步骤以及每个方法的潜在改进.
  • 讨论每个方法的优缺点,包括成本,时间,准确性和灵敏度.

主要成果:

  • 各种PHA量化方法的详细描述,包括它们的操作原则.
  • 对不同技术的优缺点进行比较分析.
  • 确定需要进一步发展方法和标准化的领域.

更多相关视频

Quantification of Polybutylene Adipate Terephthalate-based Micro- and Nano-plastics from Soil Using Proton Nuclear Magnetic Resonance Spectroscopy
05:05

Quantification of Polybutylene Adipate Terephthalate-based Micro- and Nano-plastics from Soil Using Proton Nuclear Magnetic Resonance Spectroscopy

Published on: June 6, 2025

342
Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
08:14

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste

Published on: July 18, 2025

283

相关实验视频

Last Updated: Sep 12, 2025

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K
Quantification of Polybutylene Adipate Terephthalate-based Micro- and Nano-plastics from Soil Using Proton Nuclear Magnetic Resonance Spectroscopy
05:05

Quantification of Polybutylene Adipate Terephthalate-based Micro- and Nano-plastics from Soil Using Proton Nuclear Magnetic Resonance Spectroscopy

Published on: June 6, 2025

342
Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
08:14

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste

Published on: July 18, 2025

283

结论:

  • 选择最佳的PHA量化方法取决于具体的研究目标和资源的可用性.
  • 标准化PHA测量的最佳实践对于可靠和可重复的科学结果至关重要.
  • 未来的研究应该专注于开发更有效,更敏感,更具成本效益的PHA量化方法.