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Sampling Methods: Sample Types

Sampling Methods: Sample Types

Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...

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Updated: Sep 12, 2025

Breath Collection from Children for Disease Biomarker Discovery

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

系统地比较线下呼吸采样方法.

Mark Woollam^1,2, Andrew Christensen³, Eray Schulz^4,3

¹Department of Chemistry & Chemical Biology, Indiana University Indianapolis, Indianapolis, IN, 46202, USA. mwoollam@iu.edu.

Analytical and bioanalytical chemistry

|August 5, 2025

概括

在Tedlar袋中标准化呼出的挥发性有机化合物 (VOC) 采样,提高了医疗诊断的可重复性和灵敏性. 这种方法与现有技术有很强的一致性,支持生物标志物发现.

关键词:

呼出的气息是可以呼吸的.气色谱 - 质谱学 (GCMS) 气色谱方法标准化标准化方法标准化热溶解溶解是一种热溶解.挥发性有机化合物 (VOC) 是一种挥发性有机化合物.

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Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

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Published on: June 11, 2014

相关实验视频

Last Updated: Sep 12, 2025

Breath Collection from Children for Disease Biomarker Discovery

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

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Published on: March 9, 2018

Fast and Accurate Exhaled Breath Ammonia Measurement

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Published on: June 11, 2014

科学领域:

生物医学工程生物医学工程
分析化学是一种分析化学.
医学诊断医学诊断医学诊断

背景情况:

呼气分析提供了一种非侵入性方法来检测疾病的生物标志物.
目前的局限性包括缺乏对挥发性有机化合物 (VOC) 标准化的离线采样技术.

研究的目的:

为了使线下呼吸采样标准化,使用Tedlar袋进行挥发性有机化合物 (VOC) 分析.
为了评估影响VOC异质性的因素在呼吸采集期间.
为了将Tedlar袋的性能与ReCIVA系统进行比较.

主要方法:

在Tedlar袋中影响VOC的研究因素:管道,袋子材料,分成,体积和流量.
采集了三名健康志愿者的呼吸样本,使用了Tedlar袋和ReCIVA.
通过热溶解气体染色学质谱法 (TD-GC-MS) 分析样品.

主要成果:

在志愿者中确定了15个可靠的呼吸VOC.
与ReCIVA相比,Tedlar袋对大多数检测到的VOC具有更高的敏感性.
泰德拉袋的可复制性明显高于ReCIVA (相对标准偏差较低) (p < 0.03).
在Tedlar袋和ReCIVA方法之间观察到强烈的相关性 (R2 = 0.70).

结论:

标准化Tedlar袋采样为线下VOC采集提供了可重现和敏感的方法.
泰德拉袋和ReCIVA都适合在呼气中发现挥发性有机化合物 (VOC) 生物标志物.