通过Bacillus subtilis的表面显示改善了红素过氧化酶的氧化稳定性
Na Shi1, De Cheng1, Yong Chen1
1School of Life Sciences, Jiangsu University, Jiangsu, 212013, Zhenjiang, China.
Biotechnology letters
|August 5, 2025
概括
我们使用Bacillus subtilis CotB蛋白设计了一种表面显示的红素过氧化酶 (LiP). 这种固定酶表现出增强的稳定性和活性,改善了其在环境和农业环境中对素降解的工业应用.
科学领域:
- 生物技术是生物技术.
- 酵素工程是什么意思 酵素工程
- 环境微生物学 环境微生物学
背景情况:
- 氨酸过氧化酶 (LiP) 对于氨酸降解至关重要,但在真菌生产和酶稳定性方面面临限制.
- 的工业应用受到低酶生产能力和本地真菌宿主不稳定的阻碍.
研究的目的:
- 为了克服天然LiP生产和稳定性的局限性.
- 开发一种固定式LiP,具有更好的工业应用性.
- 为了提高酶的稳定性和扩大应用潜力.
主要方法:
- 将LiP基因与Bacillus subtilis子外套蛋白CotB.融合在一起.
- 在细菌表面显示化蛋白质以固定.
- 描述了固定LiP的酶性质.
主要成果:
- 固定化LiP (CotB-LiP) 在50°C和pH值5.0时表现出最佳活性,这是从自由LiP的40°C和pH值6.0时的转变.
- 在70°C化6小时后,CotB-LiP保持了42.7%的活性,而自由LiP保持了<10%.
- 在1mM H2O2中1小时后,CotB-LiP保持了60%的相对活性,而自由LiP几乎被禁用了.
结论:
- 与自由LiP相比,在子表面显示的LiP表现出显著增强的稳定性和活性.
- 这种固定酶在环境修复和农业应用方面具有巨大的潜力.
- 该战略为低成本固定酶生产提供了一个参考.
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