转录终结促进了紧的基因组中的拼接效率和忠实性
Keaton Barr1, Kevin L He1, Andreas J Krumbein1
1Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles CA 90095-1569.
概括
在酵母中,拼接和裂变和多化 (CPA) 并没有广泛地结合在一起. 然而,CPA介导的转录终止对于保持紧基因组的拼接精度和效率至关重要.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 单核生物基因表达的表达方式
背景情况:
- 终端内子的剪接与哺乳动物的3'-end处理 (裂变和多化,CPA) 有关.
- 在真核生物之间这种合的保护仍然在很大程度上是未知的.
研究的目的:
- 在酵母模型生物*Saccharomyces cerevisiae*中研究拼接和CPA之间的功能合.
- 为了确定CPA失活是否会影响拼接效率和保真度.
主要方法:
- 在*Saccharomyces cerevisiae*中长时间读取RNA测序.
- 拼接和CPA无活化效应的分析.
- 对转录终止和读透转录的评估.
主要成果:
- 拼接无活化对酵母中CPA的影响很小.
- CPA无活化对拼接效率的影响有限,主要是由于转录终结缺陷.
- 转录终止缺陷导致了读透转录,抑制了下游拼接,并促进了加密拼接位置的使用.
结论:
- 在*Saccharomyces cerevisiae*中,剪接和CPA之间没有广泛的合.
- 有效的CPA介导的转录终止对于在酵母菌的紧基因组中的拼接忠实性和效率至关重要.
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