Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Machine learning prediction of axillary lymph node metastasis using multimodal ultrasound in breast cancer.

Future oncology (London, England)·2026
Same author

Association of Interleukin 17 Polymorphisms and IL17A Levels with Papillary Thyroid Carcinoma: A Hospital-Based Study in the Chinese Population.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research·2026
Same author

Predicting immune-related thyroiditis using polygenic risk scores in patients with advanced melanoma.

Journal for immunotherapy of cancer·2026
Same author

Development and properties of composite coatings for surface repair of cement concrete pavements.

Scientific reports·2026
Same author

Dual inhibition of glycolysis and epigenetics via a nanodelivery system for colorectal cancer treatment.

Nanomedicine : nanotechnology, biology, and medicine·2026
Same author

Ploidy shapes gemcitabine response through altered potency and delayed cell death.

bioRxiv : the preprint server for biology·2026
Same journal

A computational method to design broad-spectrum T cell-inducing vaccines applied to Betacoronaviruses.

Cell reports methods·2026
Same journal

MalDeepSeq panel: A targeted ultra-deep sequencing approach to trace drug resistance markers in Plasmodium falciparum.

Cell reports methods·2026
Same journal

Induced pluripotent stem cell-derived macrophages enable broad modeling of human inflammasome signaling.

Cell reports methods·2026
Same journal

Rapid discovery of cell-surface glycosylation regulators using a lectin-based magnetic CRISPR screen.

Cell reports methods·2026
Same journal

A real-time FRET ubiquitin transfer assay for quantitative characterization of ternary complexes in targeted protein degradation.

Cell reports methods·2026
Same journal

A high-throughput, end-to-end pipeline for extracellular miRNA biomarker discovery from human biofluids.

Cell reports methods·2026
查看所有相关文章

相关实验视频

Updated: Jun 20, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K

智能Impute是一个针对单细胞转录组数据的定向归算框架.

Sijie Yao1, Tingyi Li1, Joshua T Davis1

  • 1Department of Biostatistics and Bioinformatics, H. Lee Moffitt Cancer Center & Research Institution, Tampa, FL 33612, USA.

Cell reports methods
|August 5, 2025
PubMed
概括
此摘要是机器生成的。

智能Impute通过准确地归纳缺少的基因表达数据来增强单细胞RNA测序 (scRNA-seq) 分析,改善细胞类型识别和疾病洞察力. 这种有针对性的归算框架对于大型数据集来说是高效和可扩展的.

关键词:
CP:计算生物学 计算机生物学细胞类型的注释.放弃 放弃 放弃 放弃生成性的对抗性网络.一个单细胞RNA测序.有针对性的归算.

更多相关视频

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.7K
Reusable Single Cell for Iterative Epigenomic Analyses
10:28

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

1.4K

相关实验视频

Last Updated: Jun 20, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.7K
Reusable Single Cell for Iterative Epigenomic Analyses
10:28

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

1.4K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • 单细胞RNA测序 (scRNA-seq) 生成高维数据,缺少值,使下游分析复杂化.
  • 准确的缺失数据归算对于理解细胞异质性和生物过程至关重要.
  • 现有的归算方法可能无法保存生物细微差别或有效地扩展到大型数据集.

研究的目的:

  • 引入SmartImpute,这是一个用于scRNA-seq数据的新型归算框架.
  • 通过专注于标记基因来提高归算中的生物相关性和计算效率.
  • 提高scRNA-seq数据分析的准确性,包括聚类,细胞类型注释和轨迹推断.

主要方法:

  • 开发SmartImpute,一个使用修改后的生成对抗性推算网络 (GAIN) 的目标推算框架.
  • 整合一个多任务区分器,以赋值缺失的值,同时保留真正的生物零.
  • 适用于各种scRNA-seq数据集 (头角状细胞癌,骨髓,肺癌) 和空间转录组学数据.

主要成果:

  • 在多个scRNA-seq数据集中,SmartImpute在聚类,细胞类型注释和轨迹推断方面表现得更好.
  • 该框架成功扩展到处理超过100万个单元的数据集.
  • 对空间转录组学数据的应用揭示了增强的空间基因表达模式和聚类.

结论:

  • 在scRNA-seq和空间转录学中,SmartImpute提供了一个强大的和高效的解决方案,用于赋值缺失的数据.
  • 专注于标记基因的有针对性的方法提高了生物解释性和分析结果.
  • 智能Impute促进了对细胞异质性,疾病进展和空间转录组数据组织的更深入的洞察.