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相关概念视频

CRISPR01:59

CRISPR

52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.4K

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相关实验视频

Updated: Sep 12, 2025

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

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使用CRISPR-RfxCas13d系统对功能性外表突破异型进行系统性选.

Qiang Sun1, Xuejie Ma2, Qianqian Ning3

  • 1Cancer Institute, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China; Center of Clinical Oncology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, Jiangsu, China; Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China; Center for RNA Medicine, the Fourth Affiliated Hospital of School of Medicine, International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu 320000, Zhejiang, China.

Cell systems
|August 5, 2025
PubMed
概括

这项研究引入了一种新的CRISPR工具,以使特定的癌症驱动基因变异沉默. 研究人员确定了像HMGN3 Δ6这样的外显子跳转事件,这对结直肠癌的生长和进展至关重要.

关键词:
通过CRISPR-RfxCas13d进行查.在HMGN3Δ6结肠直肠癌是什么意思突出跳过的突出跳过

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Last Updated: Sep 12, 2025

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 癌症研究 癌症研究

背景情况:

  • 异子跳转 (ES) 是癌症中常见的剪接变化,但其功能影响尚不清楚.
  • 了解ES衍生异型对于癌症生物学和治疗开发至关重要.

研究的目的:

  • 开发一个转录特定的基因沉默平台,用于研究ES事件.
  • 为了确定驱动结直肠癌 (CRC) 进展的功能性ES事件.

主要方法:

  • 开发了一个CRISPR-RfxCas13d系统,用于针对外子-外子结.
  • 为3,744个人类ES事件创建了一个转录组范围的指导RNA库.
  • 在CRC细胞中进行功能丧失查 (体外和体内).

主要成果:

  • 确定了对CRC细胞生长至关重要的多个ES事件.
  • 发现的ES异型HMGN3 Δ6显著增强了瘤的扩散.
  • 验证了HMGN3 Δ6在CRC进展中的致癌作用.

结论:

  • CRISPR-RfxCas13d对于异构体特定的功能基因组学是有效的.
  • 来自ES的转录代表了瘤生物学的一个重要且未被充分研究的层.
  • 像HMGN3 Δ6这样的ES事件是癌症的潜在治疗标.