相关实验视频

Updated: Sep 12, 2025

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

评估活体光共聚焦显微镜用于儿科手术中的手术内诊断:可行性研究

Donatella Di Fabrizio¹, Francesca Mastroberti¹, Irene Tavolario¹

¹Pediatric Surgery Unit, Salesi Children's Hospital, Polytechnic University of Marche, Ancona, Italy.

European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie

|August 5, 2025

概括

活体光共聚焦显微镜 (FCM) 为诊断儿科瘤病理提供了高准确度. 这种快速成像技术显示出出色的灵敏度和特异性,有可能改善外科诊断.

科学领域:

医疗成像医学成像
在瘤学瘤学.
儿科病理学儿科病理学

背景情况:

活体光共聚焦显微镜 (FCM) 是一种新兴技术,用于切除组织的实时高分辨率成像.
传统的组织学需要广泛的准备,导致更长的诊断周转时间.

研究的目的:

为了评估FCM的诊断性能与传统组织学相比,在怀疑有瘤病理的儿科患者中.
评估FCM的准确性,敏感性,特异性和观察者之间的协议.

主要方法:

使用FCM分析了怀疑患有瘤病变的18个儿科组织样本.
结果与传统组织学作为黄金标准进行了比较.
计算了包括灵敏度,特异性,PPV,NPV和科恩卡帕在内的诊断指标.

主要成果:

FCM实现了100%的灵敏度和87.5%的特异性.
积极和消极的预测值分别为90.9%和100%.
在FCM和组织学之间发现了优异的一致性 (科恩的kappa=0.89),观察者之间的完全一致性 (kappa=1.00).

结论:

在儿科样本中,FCM表现出异常的诊断准确性.
该技术提供了快速,可靠的手术内结果,作为一个有价值的辅助器,对他的病理学.
需要进一步的研究来证实这些发现,并将FCM纳入临床实践.

更多相关视频

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

Published on: September 11, 2013

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

Published on: January 19, 2018

相关概念视频

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...