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使用集群定期间隔的短Palindromic重复/CRISPR相关蛋白和Argonaute核酶进行病毒检测

Liang Xu¹, Xuping Wu¹

¹The Precision Medical Center, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing 210003, China.

Clinica chimica acta; international journal of clinical chemistry

|August 5, 2025

概括

CRISPR/Cas和Argonaute (Ago) 系统提供快速,灵敏和特定的病毒检测,克服了PCR等传统方法的局限性. 这些先进的核酸检测技术有望为全球传染病控制提供可访问的诊断.

科学领域:

分子生物学分子生物学
生物技术是生物技术.
传染病诊断传染病诊断传染病诊断

背景情况:

诸如COVID-19之类的病毒性流行病突显了对高效诊断工具的迫切需要.
像聚合酶链反应 (PCR) 这样的传统方法耗时,需要专门的设备.
新兴的集群定期间隔的简短的Palindromic重复 (CRISPR) /CRISPR相关蛋白 (Cas) 和Argonaute (Ago) 系统提供快速,敏感和特定的核酸检测.

研究的目的:

审查CRISPR/Cas (Cas9,Cas12,Cas13) 和Ago系统的机制和功能.
调查这些系统在单个和多重病毒检测中的应用.
讨论CRISPR/Cas和基于ago的诊断的当前挑战和未来方向.

主要方法:

关于CRISPR/Cas和Ago系统用于病毒检测的科学文献的综述.
对特定检测平台的分析,包括DACD/RACD,DETECTR,CFNS,手机检测,MiCaR,PAND,SHERLOCKv2和CARMEN等.
讨论系统机制,灵敏度,特异性和多重复合能力.

主要成果:

通过CRISPR/Cas和Ago系统,可以达到高特异性的单拷贝检测极限.
单一检测的例子包括DACD/RACD,DETECTR,CFNS和无放大功率的手机测试.
多个复数检测的例子包括MiCaR (30个复数),PAND (5个复数),SHERLOCKv2 (4个复数) 和CARMEN (>100个目标).

结论:

基于CRISPR/Cas和ago的检测方法比传统技术具有显著的优势.
挑战包括原始空间器相邻动机 (PAM) 要求,核酸处理时间和组件稳定性.
未来在特异性,多重化和临床检测 (POCT) 方面的进展将加强全球传染病监测和控制.

关键词:

阿尔戈诺特 (Ago) 系统集群定期间隔的短时间的Palindromic重复 (CRISPR) /CRISPR相关蛋白质 (Cas) 系统.多重核酸检测多重核酸检测在核酸检测中优先检测病毒病原体这就是SARS-CoV-2病毒.通过SNP歧视性病毒检测.

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