相关实验视频

Updated: Jun 24, 2026

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

在固态发酵下,Paramyrothecium roridum增强了乳糖的产生:在中性pH脱和贝的价值化中应用

Fatemeh Kiaei¹, Ahmad Reza Shahverdi¹, Mohammad-Reza Delnavazi²

¹Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 1417614411, Iran.

International journal of biological macromolecules

|August 5, 2025

概括

这项研究优化了真菌乳糖酶的产生,以实现高效,环保的素脱聚合. 固态发酵和一种新的净化方法产生了一种强大的酶,用于将生物质分解为有价值的化合物.

科学领域:

生物技术和生物化学
环境科学环境科学
工业微生物学工业微生物学

背景情况:

乳酶酶是氨酸脱聚合的关键生物催化剂.
优化真菌乳糖酶的生产是可持续生物质加工的关键.
目前的脱方法往往涉及恶劣的化学品和条件.

研究的目的:

为了分离和表征一种土壤传播的乳糖酶产生真菌,Paramyrothecium roridum.
通过浸泡 (SmF) 和固态发酵 (SSF) 来优化乳糖生产.
为了净化和表征乳糖酶,用于其在素脱聚合过程中的应用.

主要方法:

隔离和表征Paramyrothecium. roridum. 的一种植物.
通过SSF和SmF优化乳糖生产.
使用一种新型合成亲和力矩阵 (DHBA@NH2@SiO2@FeAl2O4) 进行净化.
使用纯化的乳糖酶和1-基子 (HBT) 进行子的酶去化.

主要成果:

在优化条件下,SSF与SmF相比,乳糖酶活性增加了2倍 (50.64 U g-1).
通过使用新型亲和力矩阵,实现了4.9倍的laccase净化.
净化的乳糖酶在广泛的pH范围 (4-11) 中表现出高活性和稳定性.
酶去化达到60.8%的效率,产生有价值的副产品,如甲基醇和酸.

结论:

帕拉米罗 (Paramyrothecium roridum) 是一种可用于工业应用的真菌乳糖酶的有希望的来源.
固态发酵优于浸泡发酵,用于生产乳糖.
酶去化提供了一种环保和高效的方法,用于纤维素生物质的价值化.

关键词:

脱化是一种脱的过程.酶生产酶生产这种真菌是 laccase 菌.帕拉米罗 (Paramyrothecium roridum) 是一种类型的植物.固态发酵发酵的固态发酵方式

更多相关视频

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Published on: May 16, 2022

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

相关概念视频

Production of Alcohol

Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...

Production of Organic Acids

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...