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FGF受体通过Vangl2氨酸酸化调节神经ectoderm中的平面细胞极性
Ilya Chuykin1, Sergei Y Sokol2
1Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, USA.
Nature communications
|August 5, 2025
概括
纤维细胞生长因子受体1 (FGFR1) 信号影响脊椎动物神经管发育期间平面细胞极性. FGFR1酸化Vangl2,这是细胞定向中的关键蛋白质,揭示了形态发生过程中必不可少的交叉对话.
科学领域:
- 发展生物学 发展生物学
- 细胞信号传递 细胞信号传递
- 分子生物学分子生物学
背景情况:
- 纤维细胞生长因子受体 (FGFRs) 对胚胎发育和形态发生至关重要.
- 平面细胞极性 (PCP) 信号指导组织内的细胞方向,对于脊椎动物神经管闭合至关重要.
研究的目的:
- 为了调查FGFR信号传递和PCP在Xenopus神经形成过程中的机制联系.
- 为了阐明FGFR1活动如何影响Vangl2,一个核心PCP蛋白.
主要方法:
- 在Xenopus胚胎中FGFR1的耗尽,以观察对神经ectoderm平面极性的影响.
- 同免疫沉试验用于研究FGFR1.1对Vangl2的相互作用和酸化.
- 在Xenopus和小鼠胚胎干细胞中分析Vangl2突变 (非酸化和模仿性).
主要成果:
- 在Xenopus neuroectoderm中,FGFR1的枯竭导致异常平面极性.
- FGFR1直接与Vangl2在特定的氨酸残留物中结合并酸化.
- 由FGFR1对Vangl2的酸化影响其与PTK7和Prickle的相互作用,影响平面极性.
结论:
- 通过Vangl2氨酸酸化,FGFR1信号通路直接与平面细胞极性通路相交.
- 这种交叉对话从Xenopus保存到哺乳动物,突出了它在脊椎动物发育中的基本作用.
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