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Updated: Sep 12, 2025

Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System
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Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

Published on: May 3, 2018

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BlurryScope 通过对模糊图像进行深度学习,实现了用于 HER2 评分的紧,具有成本效益的扫描显微镜

Michael John Fanous1, Christopher Michael Seybold2, Hanlong Chen1,3,4

  • 1Electrical and Computer Engineering Department, University of California, Los Angeles, 90095, CA, USA.

NPJ digital medicine
|August 5, 2025
PubMed
概括

我们创建了BlurryScope,这是一款低成本,紧的显微镜,使用人工智能进行组织分析. 它在乳腺癌样本中自动进行HER2评分,准确度高,与昂贵的扫描仪相匹配.

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A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells10:37

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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