抑制功效,生产动态和乳酸细菌后生物的特征
Md Moklesur Rahman1,2, Awis Qurni Sazili3,4, Siti Aqlima Ahmad5
1Halal Product Research Institute, Universiti Putra Malaysia, UPM Serdang, 43400, Malaysia.
BMC microbiology
|August 5, 2025
概括
来自乳酸细菌 (LAB) 的后生物体对食源性病原体表现出广泛的抗菌活性. 这些天然化合物具有稳定性和有效性,具有作为食品防腐剂的潜力.
科学领域:
- 微生物学与食品科学 微生物学与食品科学
- 生物技术和生物活性化合物
背景情况:
- 抗菌素耐药性 (AMR) 是一个主要的全球健康和食品安全问题.
- 乳酸细菌 (LAB) 生产后生物质,后生物质是具有防腐剂潜力的生物活性化合物.
研究的目的:
- 评估来自五种LAB菌株的后生物质的抗菌谱,生产动态和物理化学特性.
- 评估LAB衍生后生物剂作为天然抗菌剂的稳定性和有效性.
主要方法:
- 评估了后生物制剂对阳性和阴性病原体的抗菌活性.
- 部分净化的类似细菌素的抑制物质 (BLIS) 使用硫酸沉和酶特性.
- 通过SDS-PAGE确定分子量,并通过温度,pH和盐度评估后生物稳定性.
主要成果:
- 来自L. plantarum和L. acidophilus的后生物体显示出显著的抗菌活性,特别是对大肠杆菌,S. Typhimurium和S. aureus.
- 后生物产量在化24-36小时之间达到峰值,抑制率为85.71-89.28%.
- 后生物在高温 (121°C),广泛的pH范围 (3-5,9-11) 和不同盐度下表现出稳定性. 解热化增强了化合物度和稳定性.
结论:
- 由LAB衍生的后生物,包括BLIS,乳酸和油酸,是稳定的,有效的天然抗菌剂.
- 这些后生物体显示出作为食品应用中的天然防腐剂的巨大潜力.
- 这些后生物质的全面表征为食品保存策略提供了宝贵的见解.
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